GIT2 Monoclonal antibody, PBS Only

Host / Isotype

Mouse / IgG1

Reactivity

Human, rat

Applications

WB,Indirect ELISA,IHC

Conjugate

Unconjugated

CloneNo.

2D9C5

Cat No : 67858-1-PBS

Synonyms

ARF GAP GIT2, CAT 2, CAT2, DKFZp686G01261, GIT2, GRK interacting protein 2, KIAA0148

WB analysis using 67858-1-Ig (same clone as 67858-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 67858-1-Ig (GIT2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 67858-1-PBS in a different storage buffer formulation.
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Various lysates were subjected to SDS PAGE followed by western blot with 67858-1-Ig (GIT2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 67858-1-PBS in a different storage buffer formulation.
IHC staining of human prostate cancer using 67858-1-Ig (same clone as 67858-1-PBS)
Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 67858-1-Ig (GIT2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67858-1-PBS in a different storage buffer formulation.

Size

67858-1-PBS targets GIT2 in WB, IHC, Indirect ELISA applications and shows reactivity with Human, rat samples.

Tested Applications	WB,Indirect ELISA,IHC Application Description
Tested Reactivity	Human, rat
Immunogen	GIT2 fusion protein Ag0321 种属同源性预测
Host / Isotype	Mouse / IgG1
Class	Monoclonal
Type	Antibody
Full Name	G protein-coupled receptor kinase interacting ArfGAP 2
Synonyms	ARF GAP GIT2, CAT 2, CAT2, DKFZp686G01261, GIT2, GRK interacting protein 2, KIAA0148
Calculated Molecular Weight	95 kDa
Observed Molecular Weight	70-85 kDa
GenBank Accession Number	BC001379
Gene Symbol	GIT2
Gene ID (NCBI)	9815
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein G purification
UNIPROT ID	Q14161
Storage Buffer	PBS Only
Storage Conditions	Store at -80°C.