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GIT2 Monoclonal antibody

GIT2 Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

2D9C5

Cat no : 67858-1-Ig

Print datasheet

Synonyms

ARF GAP GIT2, CAT 2, CAT2, DKFZp686G01261, GIT2, GRK interacting protein 2, KIAA0148

验证数据展示

  • WB analysis using 67858-1-Ig

    WB analysis using 67858-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67858-1-Ig (GIT2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

  • IHC staining of human prostate cancer using 67858-1-Ig

    IHC staining of human prostate cancer using 67858-1-Ig

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 67858-1-Ig (GIT2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inA549 cells, K-562 cells, HSC-T6 cells, HEK-293 cells, HeLa cells
Positive IHC detected inhuman prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67858-1-Ig targets GIT2 in WB, IHC, ELISA applications and shows reactivity with Human, rat samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity Human, rat
Immunogen GIT2 fusion protein Ag0321 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name G protein-coupled receptor kinase interacting ArfGAP 2
Synonyms ARF GAP GIT2, CAT 2, CAT2, DKFZp686G01261, GIT2, GRK interacting protein 2, KIAA0148
Calculated Molecular Weight 95 kDa
Observed Molecular Weight 70-85 kDa
GenBank Accession NumberBC001379
Gene Symbol GIT2
Gene ID (NCBI) 9815
RRIDAB_2918616
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ14161
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for GIT2 antibody 67858-1-IgDownload protocol
IHC protocol for GIT2 antibody 67858-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols