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CoraLite® Plus 488-conjugated GIT2 Monoclonal antibody

GIT2 Monoclonal Antibody for FC (Intra)

Host / Isotype

Mouse / IgG1

Reactivity

Human, rat

Applications

FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

2D9C5

Cat no : CL488-67858

Print datasheet

Synonyms

GIT2, GRK-interacting protein 2, KIAA0148, 2D9C5, ARF GAP GIT2, ARF GTPase-activating protein GIT2, CAT 2, CAT2, CAT-2, Cool-interacting tyrosine-phosphorylated protein 2, G protein-coupled receptor kinase-interactor 2

验证数据展示

  • FC experiment of HeLa using CL488-67858

    FC experiment of HeLa using CL488-67858

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human GIT2 (CL488-67858, Clone:2D9C5) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive FC (Intra) detected inHeLa cells
Positive FC detected inHeLa cells
For other applications, we recommend the unconjugated version of this antibody, 67858-1-Ig

推荐稀释比

ApplicationDilution
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-67858 targets GIT2 in FC (Intra) applications and shows reactivity with Human, rat samples.

Tested Applications FC (Intra) Application Description
Tested Reactivity Human, rat
Immunogen GIT2 fusion protein Ag0321 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name G protein-coupled receptor kinase interacting ArfGAP 2
Synonyms GIT2, GRK-interacting protein 2, KIAA0148, 2D9C5, ARF GAP GIT2, ARF GTPase-activating protein GIT2, CAT 2, CAT2, CAT-2, Cool-interacting tyrosine-phosphorylated protein 2, G protein-coupled receptor kinase-interactor 2
Calculated Molecular Weight 95 kDa
Observed Molecular Weight 70-85 kDa
GenBank Accession NumberBC001379
Gene Symbol GIT2
Gene ID (NCBI) 9815
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ14161
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
FC protocol for CL Plus 488 GIT2 antibody CL488-67858Download protocol
Standard Protocols
Click here to view our Standard Protocols