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  • Featured Product
  • KD/KO Validated

GKAP1 Polyclonal antibody

GKAP1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA and More (1)

Conjugate

Unconjugated

Cat no : 11395-1-AP

Print datasheet

Synonyms

FKSG21, G kinase anchoring protein 1, GKAP1, GKAP42

验证数据展示

  • WB analysis of HEK-293 using 11395-1-AP

    WB analysis of HEK-293 using 11395-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 11395-1-AP (GKAP1 Antibody) at dilution of 1:600 incubated at 4 degree celsius over night.

  • IHC staining of human testis using 11395-1-AP

    IHC staining of human testis using 11395-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 11395-1-AP (GKAP1 Antibody) at dilution of 1:50 (under 40x lens).

  • IHC staining of human cervical cancer using 11395-1-AP

    IHC staining of human cervical cancer using 11395-1-AP

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 11395-1-AP (GKAP1 Antibody) at dilution of 1:50 (under 40x lens).

经过测试的应用

Positive WB detected inHEK-293 cells, mouse testis tissue
Positive IHC detected inhuman cervical cancer tissue, human testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

11395-1-AP targets GKAP1 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsWB, IF, IHC
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Immunogen GKAP1 fusion protein Ag1914 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name G kinase anchoring protein 1
Synonyms FKSG21, G kinase anchoring protein 1, GKAP1, GKAP42
Calculated Molecular Weight 366 aa, 42 kDa
Observed Molecular Weight 45-50 kDa
GenBank Accession NumberBC014476
Gene Symbol GKAP1
Gene ID (NCBI) 80318
RRIDAB_2877761
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ5VSY0
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

GKAP1, or GKAP42, is a novel male germ cell-specific 42-kDa protein isolated by Yuasa K et al. GKAP42 mRNA is specifically expressed in testis, where it is restricted to the spermatocytes and early round spermatids.

实验方案

Product Specific Protocols
WB protocol for GKAP1 antibody 11395-1-APDownload protocol
IHC protocol for GKAP1 antibody 11395-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouse,humanWB,IF,IHC

Mol Reprod Dev

Functional role of GKAP1 in the regulation of male germ cell spontaneous apoptosis and sperm number.

Authors - Hanshu Wang
  • KO Validated
View Article