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GLI2-Specific Recombinant antibody

GLI2-Specific Uni-rAbTM Recombinant Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

241049C10

Cat no : 84141-1-RR

Print datasheet

Synonyms

Gli2, Tax helper protein, GLI Kruppel family member GLI2, Gli 2, AW546128

验证数据展示

  • WB analysis using 84141-1-RR

    WB analysis using 84141-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84141-1-RR (GLI2-Specific antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IF Staining of HepG2 using 84141-1-RR

    IF Staining of HepG2 using 84141-1-RR

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using GLI2-Specific antibody (84141-1-RR, Clone: 241049C10 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

经过测试的应用

Positive WB detected inHeLa cells, SKOV-3 cells, HT-1080 cells, A549 cells, NIH/3T3 cells
Positive IF/ICC detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy GLI2 Ready-To-Use IHC Kit. GLI2 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84141-1-RR targets GLI2-Specific in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IF/ICC, ELISA Application Description
Tested Reactivity human, mouse
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name GLI family zinc finger 2
Synonyms Gli2, Tax helper protein, GLI Kruppel family member GLI2, Gli 2, AW546128
Calculated Molecular Weight 168 kDa
Observed Molecular Weight80-90 kDa, 130-140 kDa, 160-180 kDa
GenBank Accession NumberNM_005270
Gene Symbol GLI2
Gene ID (NCBI) 2736
RRIDAB_3671702
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ0VGT2
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

GLI2, also named as THP, belongs to the GLI C2H2-type zinc-finger protein family. GLI2 play a role during embryogenesis. It binds to the DNA sequence 5'-GAACCACCCA-3' which is part of the TRE-2S regulatory element that augments the Tax-dependent enhancer of human T-cell leukemia virus type 1. It is implicated in the transduction of SHH signal. Mutation of GLI2 will cause of holoprosencephaly type 9 (HPE9). Four possible isoforms (hGli2a, b, g, and d) are formed by combinations of two independent alternative splicings, and all the isoforms could bind to a DNA motif, TRE2S. hGli2 a, b, g, and d. These isoforms encode 133-, 131-, 88-, and 86-kDa proteins, respectively.

实验方案

Product Specific Protocols
WB protocol for GLI2-Specific antibody 84141-1-RRDownload protocol
IF protocol for GLI2-Specific antibody 84141-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols