CoraLite® Plus 488-conjugated GLI2-Specific Recombinant antibody
GLI2-Specific Uni-rAbTM Recombinant Antibody for IF/ICC
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse
Applications
IF/ICC
Conjugate
CoraLite® Plus 488 Fluorescent Dye
CloneNo.
241049C10
验证数据展示
经过测试的应用
| Positive IF/ICC detected in | HepG2 cells |
Planning an IHC experiment? We recommend our IHCeasy GLI2 Ready-To-Use IHC Kit. GLI2 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 84141-1-PBS
推荐稀释比
| Application | Dilution |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL488-84141 targets GLI2-Specific in IF/ICC applications and shows reactivity with human, mouse samples.
| Tested Applications | IF/ICC Application Description |
| Tested Reactivity | human, mouse |
| Immunogen | Peptide 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Full Name | GLI family zinc finger 2 |
| Synonyms | Gli2, Tax helper protein, GLI Kruppel family member GLI2, Gli 2, AW546128 |
| Calculated Molecular Weight | 168 kDa |
| Observed Molecular Weight | 80-90 kDa, 130-140 kDa, 160-180 kDa |
| GenBank Accession Number | NM_005270 |
| Gene Symbol | GLI2 |
| Gene ID (NCBI) | 14633 |
| RRID | AB_3673343 |
| Conjugate | CoraLite® Plus 488 Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 493 nm / 522 nm |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q0VGT2 |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
GLI2, also named as THP, belongs to the GLI C2H2-type zinc-finger protein family. GLI2 play a role during embryogenesis. It binds to the DNA sequence 5'-GAACCACCCA-3' which is part of the TRE-2S regulatory element that augments the Tax-dependent enhancer of human T-cell leukemia virus type 1. It is implicated in the transduction of SHH signal. Mutation of GLI2 will cause of holoprosencephaly type 9 (HPE9). Four possible isoforms (hGli2a, b, g, and d) are formed by combinations of two independent alternative splicings, and all the isoforms could bind to a DNA motif, TRE2S. hGli2 a, b, g, and d. These isoforms encode 133-, 131-, 88-, and 86-kDa proteins, respectively.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 GLI2-Specific antibody CL488-84141 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |