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验证数据展示

  • FC experiment of HepG2 using CL594-18989

    FC experiment of HepG2 using CL594-18989

    1X10^6 HepG2 cells were intracellularly stained with 0.4 ug CoraLite®594 Anti-Mouse GLI2-Specific (CL594-18989) (red), or 0.4 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

  • Featured Product
  • KD/KO Validated

CoraLite®594-conjugated GLI2-Specific Polyclonal antibody

GLI2-Specific Polyclonal Antibody for FC (Intra)
Cat No. CL594-18989

Print datasheet

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

FC (Intra)

Synonyms

Gli2, Thp, Tax helper protein, GLI Kruppel family member GLI2, Gli 2

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite®594
Size: 

-/ -

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经过测试的应用

Positive FC (Intra) detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy GLI2 Ready-To-Use IHC Kit. GLI2 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 18989-1-AP

推荐稀释比

ApplicationDilution
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-18989 targets GLI2-Specific in FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Applications FC (Intra) Application Description
Tested Reactivity human, mouse, rat
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name GLI family zinc finger 2
Synonyms Gli2, Thp, Tax helper protein, GLI Kruppel family member GLI2, Gli 2
Calculated Molecular Weight 168 kDa
Observed Molecular Weight167 kDa， 88 kDa
GenBank Accession NumberNM_005270
Gene Symbol GLI2
Gene ID (NCBI) 2736
RRIDAB_2919850
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission Maxima Wavelengths588 nm / 604 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ0VGT2
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

GLI2, also named as THP, belongs to the GLI C2H2-type zinc-finger protein family. GLI2 play a role during embryogenesis. It binds to the DNA sequence 5'-GAACCACCCA-3' which is part of the TRE-2S regulatory element that augments the Tax-dependent enhancer of human T-cell leukemia virus type 1. It is implicated in the transduction of SHH signal. Mutation of GLI2 will cause of holoprosencephaly type 9 (HPE9). Four possible isoforms (hGli2a, b, g, and d) are formed by combinations of two independent alternative splicings, and all the isoforms could bind to a DNA motif, TRE2S. hGli2 a, b, g, and d. These isoforms encode 133-, 131-, 88-, and 86-kDa proteins, respectively.

实验方案

Product Specific Protocols
FC protocol for CL594 GLI2-Specific antibody CL594-18989Download protocol
Standard Protocols
Click here to view our Standard Protocols