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  • Featured Product
  • KD/KO Validated

CoraLite®594-conjugated GLI2-Specific Polyclonal antibody

GLI2-Specific Polyclonal Antibody for FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

FC (Intra)

Conjugate

CoraLite®594 Fluorescent Dye

Cat no : CL594-18989

Print datasheet

Synonyms

GLI2-Specific, Gli2, AW546128, Gli 2, GLI Kruppel family member GLI2, Tax helper protein, Thp, Zinc finger protein GLI2

验证数据展示

  • FC experiment of HepG2 using CL594-18989

    FC experiment of HepG2 using CL594-18989

    1X10^6 HepG2 cells were intracellularly stained with 0.4 ug CoraLite®594 Anti-Mouse GLI2-Specific (CL594-18989) (red), or 0.4 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

经过测试的应用

Positive FC (Intra) detected inHepG2 cells
Positive FC detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy GLI2 Ready-To-Use IHC Kit. GLI2 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 18989-1-AP

推荐稀释比

ApplicationDilution
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-18989 targets GLI2-Specific in FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Applications FC (Intra) Application Description
Tested Reactivity human, mouse, rat
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name GLI-Kruppel family member GLI2
Synonyms GLI2-Specific, Gli2, AW546128, Gli 2, GLI Kruppel family member GLI2, Tax helper protein, Thp, Zinc finger protein GLI2
Calculated Molecular Weight 168 kDa
Observed Molecular Weight167 kDa， 88 kDa
GenBank Accession NumberNM_001081125
Gene Symbol Gli2
Gene ID (NCBI) 14633
RRIDAB_2919850
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission Maxima Wavelengths588 nm / 604 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ0VGT2
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

GLI2, also named as THP, belongs to the GLI C2H2-type zinc-finger protein family. GLI2 play a role during embryogenesis. It binds to the DNA sequence 5'-GAACCACCCA-3' which is part of the TRE-2S regulatory element that augments the Tax-dependent enhancer of human T-cell leukemia virus type 1. It is implicated in the transduction of SHH signal. Mutation of GLI2 will cause of holoprosencephaly type 9 (HPE9). Four possible isoforms (hGli2a, b, g, and d) are formed by combinations of two independent alternative splicings, and all the isoforms could bind to a DNA motif, TRE2S. hGli2 a, b, g, and d. These isoforms encode 133-, 131-, 88-, and 86-kDa proteins, respectively.

实验方案

Product Specific Protocols
FC protocol for CL594 GLI2-Specific antibody CL594-18989Download protocol
Standard Protocols
Click here to view our Standard Protocols