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  • Featured Product
  • KD/KO Validated

KGA/GAC Recombinant antibody, PBS Only

KGA/GAC Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

4C20

Cat no : 81486-1-PBS

Print datasheet

Synonyms

GLS, KGA, GAM, GAC, EC:3.5.1.2

验证数据展示

  • WB analysis using 81486-1-RR (same clone as 81486-1-PBS)

    WB analysis using 81486-1-RR (same clone as 81486-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 81486-1-RR (GLS antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 81486-1-PBS in a different storage buffer formulation.

  • WB analysis of A549 using 81486-1-RR (same clone as 81486-1-PBS)

    WB analysis of A549 using 81486-1-RR (same clone as 81486-1-PBS)

    WB result of GLS antibody (81486-1-RR; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-GLS transfected A549 cells. This data was developed using the same antibody clone with 81486-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse brain using 81486-1-RR (same clone as 81486-1-PBS)

    IHC staining of mouse brain using 81486-1-RR (same clone as 81486-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 81486-1-RR (GLS antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81486-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with KGA/GAC antibody included
  • IF Staining of A549 using 81486-1-RR (same clone as 81486-1-PBS)

    IF Staining of A549 using 81486-1-RR (same clone as 81486-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using GLS antibody (81486-1-RR, Clone: 4C20 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 81486-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 81486-1-RR

    Affinity and Kinetic Characterization of 81486-1-RR

    Biolayer interferometry (BLl) kinetic assays of 81486-1-RR against Human GLS were performed. The affinity constant is 0.182 nM.

Planning an IHC experiment? We recommend our IHCeasy KGA/GAC Ready-To-Use IHC Kit. KGA/GAC primary antibody included.

产品信息

81486-1-PBS targets KGA/GAC in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, Indirect ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen KGA/GAC fusion protein Ag3505 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name glutaminase
Synonyms GLS, KGA, GAM, GAC, EC:3.5.1.2
Calculated Molecular Weight 669 aa, 73 kDa
Observed Molecular Weight58 kDa, 65 kDa
GenBank Accession NumberBC038507
Gene Symbol GLS
Gene ID (NCBI) 2744
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDO94925
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

GLS, also named as GLS1 and KIAA0838, belongs to the glutaminase family. It catalyzes the first reaction in the primary pathway for the renal catabolism of glutamine. Glutaminase-, glutamate-, and taurine-immunoreactive neurons develop neurofibrillary tangles in Alzheimer's disease(PMID:1672808).The glutaminase band in AA/C1 cells is more intense than in HT29 cells, in accordance with measurements of glutaminase activity, and had the same molecular mass of approx. 63 kDa. The bands for both cell lines are clearly different in size from both rat liver glutaminase (58 kDa) and rat kidney glutaminase (65 kDa)(PMID:12408749). It also reveals a molecular weight of 83-84 kDa as a phosphate-dependent glutaminase(PMID:447624;7512428). It has 3 isoforms named as KGA, GAM, GAC. This antibody can recognize KGA and GAC.