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KGA/GAC Recombinant antibody

KGA/GAC Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, IF-P, ELISA
Cat No. 81486-1-RR

产品说明书

CloneNo. 4C20

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, IF-P, ELISA and More (1)

GLS, KGA, 4C20, AAD20, EC:3.5.1.2

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 750
规格: 

-/ -


经过测试的应用

Positive WB detected inA549 cells
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inrat brain tissue, mouse brain tissue
Positive IF/ICC detected inA549 cells
Planning an IHC experiment? We recommend our IHCeasy KGA/GAC Ready-To-Use IHC Kit. KGA/GAC primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)-PIF-P : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

81486-1-RR targets KGA/GAC in WB, IHC, IF/ICC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, IF-P, ELISA Application Description
文献引用应用WB, IHC, IF
经测试反应性 human, mouse, rat
文献引用反应性human
免疫原 KGA/GAC fusion protein Ag3505 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 glutaminase
别名 GLS, KGA, 4C20, AAD20, EC:3.5.1.2
计算分子量 669 aa, 73 kDa
观测分子量58 kDa, 65 kDa
GenBank蛋白编号BC038507
基因名称 GLS
Gene ID (NCBI) 2744
RRIDAB_2935556
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDO94925
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

GLS, also named as GLS1 and KIAA0838, belongs to the glutaminase family. It catalyzes the first reaction in the primary pathway for the renal catabolism of glutamine. Glutaminase-, glutamate-, and taurine-immunoreactive neurons develop neurofibrillary tangles in Alzheimer's disease(PMID:1672808).The glutaminase band in AA/C1 cells is more intense than in HT29 cells, in accordance with measurements of glutaminase activity, and had the same molecular mass of approx. 63 kDa. The bands for both cell lines are clearly different in size from both rat liver glutaminase (58 kDa) and rat kidney glutaminase (65 kDa)(PMID:12408749). It also reveals a molecular weight of 83-84 kDa as a phosphate-dependent glutaminase(PMID:447624;7512428). It has 3 isoforms named as KGA, GAM, GAC. This antibody can recognize KGA and GAC.

实验方案

Product Specific Protocols
WB protocol for KGA/GAC antibody 81486-1-RRDownload protocol
IHC protocol for KGA/GAC antibody 81486-1-RRDownload protocol
IF protocol for KGA/GAC antibody 81486-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIF

Am J Cancer Res

IGF2BP3-mediated regulation of GLS and GLUD1 gene expression promotes treg-induced immune escape in human cervical cancer

Authors - Tiantian Zhou
humanWB

bioRxiv

IRF5 mediates adaptive immunity via altered glutamine metabolism, mTORC1 signaling and post-transcriptional regulation following T cell receptor activation

Authors - Zarina Brune
humanIHC

Front Endocrinol (Lausanne)

Hypoxia-inducible factor-2α promotes fibrosis in non-alcoholic fatty liver disease by enhancing glutamine catabolism and inhibiting yes-associated protein phosphorylation in hepatic stellate cells

Authors - Ranran Yan
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