验证数据展示
经过测试的应用
| Positive WB detected in | COLO 320 cells, mouse brain tissue |
| Positive FC (Intra) detected in | HeLa cells |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL750-81486 targets KGA/GAC in WB, FC (Intra) applications and shows reactivity with human, mouse, rat samples.
| Tested Applications | WB, FC (Intra) Application Description |
| Tested Reactivity | human, mouse, rat |
| Immunogen | KGA/GAC fusion protein Ag3505 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Full Name | glutaminase |
| Synonyms | GLS, KGA, GAM, GAC, EC:3.5.1.2 |
| Calculated Molecular Weight | 669 aa, 73 kDa |
| Observed Molecular Weight | 58 kDa, 65 kDa |
| GenBank Accession Number | BC038507 |
| Gene Symbol | GLS |
| Gene ID (NCBI) | 2744 |
| RRID | AB_3673729 |
| Conjugate | CoraLite® Plus 750 Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 755 nm / 780 nm |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | O94925 |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
GLS, also named as GLS1 and KIAA0838, belongs to the glutaminase family. It catalyzes the first reaction in the primary pathway for the renal catabolism of glutamine. Glutaminase-, glutamate-, and taurine-immunoreactive neurons develop neurofibrillary tangles in Alzheimer's disease(PMID:1672808). The glutaminase band in AA/C1 cells is more intense than in HT29 cells, in accordance with measurements of glutaminase activity, and had the same molecular mass of approx. 63 kDa. The bands for both cell lines are clearly different in size from both rat liver glutaminase (58 kDa) and rat kidney glutaminase (65 kDa)(PMID:12408749). It also reveals a molecular weight of 83-84 kDa as a phosphate-dependent glutaminase(PMID:447624;7512428). It has 3 isoforms named as KGA, GAM, GAC. This antibody can recognize KGA and GAC.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for CL Plus 750 KGA/GAC antibody CL750-81486 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |