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Species Reactivity
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验证数据展示

  • WB analysis using 67026-1-Ig (same clone as 67026-1-PBS)

    WB analysis using 67026-1-Ig (same clone as 67026-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67026-1-Ig (GLUD1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67026-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver cancer using 67026-1-Ig (same clone as 67026-1-PBS)

    IHC staining of human liver cancer using 67026-1-Ig (same clone as 67026-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67026-1-Ig (GLUD1 antibody) at dilution of 1:1000 (under 10x lens) proteolytic pre-treatment mediated antigen retrieved with Tris-EDTA buffer (pH9). This data was developed using the same antibody clone with 67026-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with GLUD1 antibody included
  • IHC staining of human liver cancer using 67026-1-Ig (same clone as 67026-1-PBS)

    IHC staining of human liver cancer using 67026-1-Ig (same clone as 67026-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67026-1-Ig (GLUD1 antibody) at dilution of 1:1000 (under 40x lens) proteolytic pre-treatment mediated antigen retrieved with Tris-EDTA buffer (pH9). This data was developed using the same antibody clone with 67026-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with GLUD1 antibody included
  • IF Staining of human liver cancer using 67026-1-Ig (same clone as 67026-1-PBS)

    IF Staining of human liver cancer using 67026-1-Ig (same clone as 67026-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using GLUD1 antibody (67026-1-Ig, Clone: 4G10D3 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67026-1-PBS in a different storage buffer formulation.

  • IF Staining of human liver cancer using 67026-1-Ig (same clone as 67026-1-PBS)

    IF Staining of human liver cancer using 67026-1-Ig (same clone as 67026-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using GLUD1 antibody (67026-1-Ig, Clone: 4G10D3 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67026-1-PBS in a different storage buffer formulation.

  • IF Staining of mouse liver using 67026-1-Ig (same clone as 67026-1-PBS)

    IF Staining of mouse liver using 67026-1-Ig (same clone as 67026-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse liver tissue using GLUD1 antibody (67026-1-Ig, Clone: 4G10D3 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67026-1-PBS in a different storage buffer formulation.

GLUD1 Monoclonal antibody, PBS Only

GLUD1 Monoclonal Antibody for WB, IHC, IF-P, Indirect ELISA
Cat No. 67026-1-PBS

产品说明书

CloneNo. 4G10D3

宿主/亚型

Mouse / IgG2b

种属反应性

human, mouse, rat

应用

WB, IHC, IF-P, Indirect ELISA

别名

GLUD, GDH1, GDH 1, GDH, EC:1.4.1.3

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy GLUD1 Ready-To-Use IHC Kit. GLUD1 primary antibody included.

产品信息

67026-1-PBS targets GLUD1 in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF-P, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 GLUD1 fusion protein Ag6179 种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 glutamate dehydrogenase 1
别名 GLUD, GDH1, GDH 1, GDH, EC:1.4.1.3
计算分子量 61 kDa
观测分子量 45-55 kDa
GenBank蛋白编号BC040132
基因名称 GLUD1
Gene ID (NCBI) 2746
RRIDAB_2882341
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP00367
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Human glutamate dehydrogenase (GDH), an enzyme central to the metabolism of glutamate, is known to exist in housekeeping and nerve tissue-specific isoforms encoded by the GLUD1 and GLUD2 genes, respectively. It catalyses the reversible inter-conversion of glutamate to alpha-ketoglutarate and ammonia, thus interconnecting amino acid and carbohydrate metabolism. GLUD1 might contribute to the formation of specific synapses in the hippocampus such as those formed by the projecting neurons of the entorhinal cortex(PMID: 22138648). GLUD1 has a calculated molecular mass of 61 kDa and an apparent molecular mass of 45-55 kDa with the 53aa transit peptide removed.