GLUD1 Monoclonal antibody
GLUD1 Monoclonal Antibody for WB, IHC, IF-P, ELISA
Host / Isotype
Mouse / IgG2b
Reactivity
Human, Mouse, Rat and More (2)
Applications
WB, IHC, IF-P, ELISA and More (1)
Conjugate
Unconjugated
CloneNo.
4G10D3
验证数据展示
经过测试的应用
Positive WB detected in | HeLa cells, HepG2 cells, L02 cells, HuH-7 cells, HSC-T6 cells, NIH/3T3 cells |
Positive IHC detected in | human liver cancer tissue, human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF-P detected in | human liver cancer tissue |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:5000-1:50000 |
Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
Immunofluorescence (IF)-P | IF-P : 1:200-1:800 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
发表文章中的应用
WB | See 2 publications below |
IF | See 1 publications below |
产品信息
67026-1-Ig targets GLUD1 in WB, IHC, IF-P, ELISA applications and shows reactivity with Human, Mouse, Rat samples.
Tested Applications | WB, IHC, IF-P, ELISA Application Description |
Cited Applications | WB, IF |
Tested Reactivity | Human, Mouse, Rat |
Cited Reactivity | human, mouse |
Immunogen | GLUD1 fusion protein Ag6179 种属同源性预测 |
Host / Isotype | Mouse / IgG2b |
Class | Monoclonal |
Type | Antibody |
Full Name | glutamate dehydrogenase 1 |
Synonyms | GDH, GDH 1, GDH1, GLUD, GLUD1, glutamate dehydrogenase 1 |
Calculated Molecular Weight | 61 kDa |
Observed Molecular Weight | 45-55 kDa |
GenBank Accession Number | BC040132 |
Gene Symbol | GLUD1 |
Gene ID (NCBI) | 2746 |
RRID | AB_2882341 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
UNIPROT ID | P00367 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
---|---|
WB protocol for GLUD1 antibody 67026-1-Ig | Download protocol |
IHC protocol for GLUD1 antibody 67026-1-Ig | Download protocol |
IF protocol for GLUD1 antibody 67026-1-Ig | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Sci Transl Med Aberrant methylmalonylation underlies methylmalonic acidemia and is attenuated by an engineered sirtuin | ||
Cell Insight Deacetylation of GLUD1 maintains the survival of lung adenocarcinoma cells under glucose starvation by inhibiting autophagic cell death |