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验证数据展示

  • IF Staining of human liver cancer using CL594-67026

    IF Staining of human liver cancer using CL594-67026

    Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using CoraLite®594-conjugated GLUD1 antibody (CL594-67026, Clone: 4G10D3 ) at dilution of 1:200.

  • IF Staining of human liver cancer using CL594-67026

    IF Staining of human liver cancer using CL594-67026

    Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using CoraLite®594-conjugated GLUD1 antibody (CL594-67026, Clone: 4G10D3 ) at dilution of 1:200.

CoraLite®594-conjugated GLUD1 Monoclonal antibody

GLUD1 Monoclonal Antibody for IF-P
Cat No. CL594-67026

产品说明书

CloneNo. 4G10D3

宿主/亚型

Mouse / IgG2b

种属反应性

Human, Mouse, Rat

应用

IF-P

别名

GDH, GDH 1, GDH1, GLUD, GLUD1, glutamate dehydrogenase 1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite®594
规格: 

-/ -

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经过测试的应用

Positive IF-P detected inhuman liver cancer tissue
Planning an IHC experiment? We recommend our IHCeasy GLUD1 Ready-To-Use IHC Kit. GLUD1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 67026-1-Ig

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-67026 targets GLUD1 in IF-P applications and shows reactivity with Human, Mouse, Rat samples.

经测试应用 IF-P Application Description
经测试反应性 Human, Mouse, Rat
免疫原 GLUD1 fusion protein Ag6179 种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 glutamate dehydrogenase 1
别名 GDH, GDH 1, GDH1, GLUD, GLUD1, glutamate dehydrogenase 1
计算分子量 61 kDa
观测分子量 45-55 kDa
GenBank蛋白编号BC040132
基因名称 GLUD1
Gene ID (NCBI) 2746
RRIDAB_2920074
偶联类型 CoraLite®594 Fluorescent Dye
最大激发/发射波长588 nm / 604 nm
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP00367
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Human glutamate dehydrogenase (GDH), an enzyme central to the metabolism of glutamate, is known to exist in housekeeping and nerve tissue-specific isoforms encoded by the GLUD1 and GLUD2 genes, respectively. It catalyses the reversible inter-conversion of glutamate to alpha-ketoglutarate and ammonia, thus interconnecting amino acid and carbohydrate metabolism. GLUD1 might contribute to the formation of specific synapses in the hippocampus such as those formed by the projecting neurons of the entorhinal cortex(PMID: 22138648). GLUD1 has a calculated molecular mass of 61 kDa and an apparent molecular mass of 45-55 kDa with the 53aa transit peptide removed.

实验方案

Product Specific Protocols
IF protocol for CL594 GLUD1 antibody CL594-67026Download protocol
Standard Protocols
Click here to view our Standard Protocols