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Anti-Human GM-CSF Rabbit Recombinant Antibody, PBS Only

GM-CSF Uni-rAbTM Recombinant Antibody for FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC (Intra)

Conjugate

Unconjugated

CloneNo.

240183C7

Cat no : 98050-1-PBS

Print datasheet

Synonyms

CSF2, CSF 2, CSF, Colony-stimulating factor, Colony Stimulating Factor 2

验证数据展示

  • FC experiment of human PBMCs using 98050-1-RR (same clone as 98050-1-PBS)

    FC experiment of human PBMCs using 98050-1-RR (same clone as 98050-1-PBS)

    1x10^6 untreated or PMA, Ionomycin and Brefeldin A treated human PBMCs were intracellularly stained with 0.25 ug Anti-Human GM-CSF Rabbit Recombinant Antibody (98050-1-RR, Clone:240183C7) and PE-conjugated Goat Anti-Rabbit IgG. Cell were then stained with CoraLite® Plus 647 Anti-Human CD4. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 98050-1-PBS in a different storage buffer formulation.

  • FC experiment of human PBMCs using 98050-1-RR (same clone as 98050-1-PBS)

    FC experiment of human PBMCs using 98050-1-RR (same clone as 98050-1-PBS)

    1x10^6 untreated (black) or PMA, Ionomycin and Brefeldin A treated human PBMCs were intracellularly stained with 0.25 ug Anti-Human GM-CSF Rabbit Recombinant Antibody (98050-1-RR, Clone:240183C7) and PE-conjugated Goat Anti-Rabbit IgG(red). 1x10^6 PMA, Ionomycin and Brefeldin A treated human PBMCs were intracellularly stained with 0.25 ug Isotype Control and PE-conjugated Goat Anti-Rabbit IgG (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 98050-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 98050-1-RR

    Affinity and Kinetic Characterization of 98050-1-RR

    Biolayer interferometry (BLl) kinetic assays of 98050-1-RR against Human GM-CSF were performed. The affinity constant is 3.46 nM.

产品信息

The immunogen of 98050-1-PBS is GM-CSF Fusion Protein expressed in E. coli.

Tested Applications FC (Intra) Application Description
Tested Reactivity human
Immunogen Fusion Protein 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name colony stimulating factor 2 (granulocyte-macrophage)
Synonyms CSF2, CSF 2, CSF, Colony-stimulating factor, Colony Stimulating Factor 2
Gene Symbol GM-CSF
Gene ID (NCBI) 1437
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP04141
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C