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Anti-Human GM-CSF Rabbit Recombinant Antibody

GM-CSF Uni-rAbTM Recombinant Antibody for FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC (Intra)

Conjugate

Unconjugated

CloneNo.

240183C7

Cat no : 98050-1-RR

Print datasheet

Synonyms

CSF2, CSF 2, CSF, Colony-stimulating factor, Colony Stimulating Factor 2

验证数据展示

  • FC experiment of human PBMCs using 98050-1-RR

    FC experiment of human PBMCs using 98050-1-RR

    1x10^6 untreated or PMA, Ionomycin and Brefeldin A treated human PBMCs were intracellularly stained with 0.25 ug Anti-Human GM-CSF Rabbit Recombinant Antibody (98050-1-RR, Clone:240183C7) and PE-conjugated Goat Anti-Rabbit IgG. Cell were then stained with CoraLite® Plus 647 Anti-Human CD4. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • FC experiment of human PBMCs using 98050-1-RR

    FC experiment of human PBMCs using 98050-1-RR

    1x10^6 untreated (black) or PMA, Ionomycin and Brefeldin A treated human PBMCs were intracellularly stained with 0.25 ug Anti-Human GM-CSF Rabbit Recombinant Antibody (98050-1-RR, Clone:240183C7) and PE-conjugated Goat Anti-Rabbit IgG(red). 1x10^6 PMA, Ionomycin and Brefeldin A treated human PBMCs were intracellularly stained with 0.25 ug Isotype Control and PE-conjugated Goat Anti-Rabbit IgG (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 98050-1-RR

    Affinity and Kinetic Characterization of 98050-1-RR

    Biolayer interferometry (BLl) kinetic assays of 98050-1-RR against Human GM-CSF were performed. The affinity constant is 3.46 nM.

经过测试的应用

Positive FC (Intra) detected inPMA, Ionomycin and Brefeldin A treated human PBMCs

推荐稀释比

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 98050-1-RR is GM-CSF Fusion Protein expressed in E. coli.

Tested Applications FC (Intra) Application Description
Tested Reactivity human
Immunogen Fusion Protein 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name colony stimulating factor 2 (granulocyte-macrophage)
Synonyms CSF2, CSF 2, CSF, Colony-stimulating factor, Colony Stimulating Factor 2
Gene Symbol GM-CSF
Gene ID (NCBI) 1437
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP04141
Storage Buffer PBS with 0.09% sodium azide.
Storage ConditionsStore at 2-8°C. Stable for one year after shipment.

背景介绍

Granulocyte-macrophage colony-stimulating factor (GM-CSF), also known as colony-stimulating factor 2 (CSF2), is a monomeric glycoprotein secreted by macrophages, T cells, mast cells, natural killer cells, endothelial cells and fibroblasts. GM-CSF is a hematopoietic growth factor that stimulates the development of early erythroid megakaryocytic and eosinophilic progenitor cells (PMID 2990035). GM-CSF stimulates stem cells to produce granulocytes (neutrophils, eosinophils, and basophils) and monocytes (PMID 3021817, 2984574, 6390681).

实验方案

Product Specific Protocols
FC protocol for GM-CSF antibody 98050-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols