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GM130;GOLGA2 Recombinant antibody, PBS Only

GM130;GOLGA2 Recombinant Antibody for WB,Indirect ELISA,IF

Host / Isotype

Rabbit / IgG

Reactivity

Human, rat

Applications

WB,Indirect ELISA,IF

Conjugate

Unconjugated

CloneNo.

1M17

Cat No : 82705-8-PBS

Print datasheet

Synonyms

GM130, GM130 autoantigen, GOLGA2, Golgin 95, Golgin subfamily A member 2

验证数据展示

  • WB analysis using 82705-8-RR (same clone as 82705-8-PBS)

    WB analysis using 82705-8-RR (same clone as 82705-8-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 82705-8-RR (GM130;GOLGA2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82705-8-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 82705-8-RR (same clone as 82705-8-PBS)

    IF Staining of HeLa using 82705-8-RR (same clone as 82705-8-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using GM130;GOLGA2 antibody (82705-8-RR, Clone: 1M17 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 82705-8-PBS in a different storage buffer formulation.

产品信息

82705-8-PBS targets GM130;GOLGA2 in WB, IF, Indirect ELISA applications and shows reactivity with Human, rat samples.

Tested Applications WB,Indirect ELISA,IF Application Description
Tested Reactivity Human, rat
Immunogen GM130;GOLGA2 fusion protein Ag1848 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name golgi autoantigen, golgin subfamily a, 2
Synonyms GM130, GM130 autoantigen, GOLGA2, Golgin 95, Golgin subfamily A member 2
Calculated Molecular Weight 111 kDa
Observed Molecular Weight130-140 kDa
GenBank Accession NumberBC014188
Gene Symbol GOLGA2
Gene ID (NCBI) 2801
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ08379
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

背景介绍

GOLGA2, also known as GM130, is a 130 kDa cis-Golgi matrix protein which is one component of the detergent and salt resistant Golgi matrix. It is a peripheral membrane protein highly bound to Golgi membrane and localized mainly at the cytoplasmic face of cis-Golgi membrane. Together with its interacting partner proteins, including p115, giantin, GRASP65, and Rab GTPase, GOLGA2/GM130 is involved in the regulation of ER-to-Golgi transport and also in the maintenance of the Golgi structure. Emerging evidence suggest that the GOLGA2/GM130 has potential roles in the control of glycosylation, cell cycle progression, and higher order cell functions such as cell polarization and directed cell migration. (PMID: 20197635)