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验证数据展示

  • FC experiment of HeLa using CL488-66080

    FC experiment of HeLa using CL488-66080

    1x10^6 HeLa cells were intracellularly stained with 0.4 ug Coralite® Plus 488 Gm2a Monoclonal Antibody (CL488-66080, Clone:1E4D3)(red), or 0.4 ug Isotype Control (CL488-66360-2) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Featured Product
  • KD/KO验证

CoraLite® Plus 488-conjugated GM2A Monoclonal antibody

GM2A Monoclonal Antibody for FC (Intra)
Cat No. CL488-66080

产品说明书

CloneNo. 1E4D3

宿主/亚型

Mouse / IgG2a

种属反应性

human

应用

FC (Intra)

别名

SAP-3, SAP3, GM2-AP, GM2AP, Ganglioside GM2 activator isoform short

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive FC (Intra) detected inHeLa cells
For other applications, we recommend the unconjugated version of this antibody, 66080-1-Ig

推荐稀释比

应用推荐稀释比
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-66080 targets GM2A in FC (Intra) applications and shows reactivity with human samples.

经测试应用 FC (Intra) Application Description
经测试反应性 human
免疫原 GM2A fusion protein Ag4394 种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 GM2 ganglioside activator
别名 SAP-3, SAP3, GM2-AP, GM2AP, Ganglioside GM2 activator isoform short
计算分子量 22 kDa
观测分子量 22 kDa
GenBank蛋白编号BC009273
基因名称 GM2A
Gene ID (NCBI) 2760
RRIDAB_3672903
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP17900
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

GM2A, also named as GM2-AP and SAP-3, is the large binding pocket which can accommodate several single chain phospholipids and fatty acids. GM2A also exhibits some calcium-independent phospholipase activity. It plays a key role in the degradation of ganglioside GM2 (GM2). GM2A stimulates only the breakdown of ganglioside GM2 and glycolipid GA2 by beta-hexosaminidase A.

实验方案

Product Specific Protocols
FC protocol for CL Plus 488 GM2A antibody CL488-66080Download protocol
Standard Protocols
Click here to view our Standard Protocols