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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated GM2A Monoclonal antibody

GM2A Monoclonal Antibody for FC (Intra)

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1E4D3

Cat no : CL488-66080

Print datasheet

Synonyms

SAP-3, SAP3, GM2-AP, GM2AP, Ganglioside GM2 activator isoform short

验证数据展示

  • FC experiment of HeLa using CL488-66080

    FC experiment of HeLa using CL488-66080

    1x10^6 HeLa cells were intracellularly stained with 0.4 ug Coralite® Plus 488 Gm2a Monoclonal Antibody (CL488-66080, Clone:1E4D3)(red), or 0.4 ug Isotype Control (CL488-66360-2) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive FC (Intra) detected inHeLa cells
For other applications, we recommend the unconjugated version of this antibody, 66080-1-Ig

推荐稀释比

ApplicationDilution
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-66080 targets GM2A in FC (Intra) applications and shows reactivity with human samples.

Tested Applications FC (Intra) Application Description
Tested Reactivity human
Immunogen GM2A fusion protein Ag4394 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name GM2 ganglioside activator
Synonyms SAP-3, SAP3, GM2-AP, GM2AP, Ganglioside GM2 activator isoform short
Calculated Molecular Weight 22 kDa
Observed Molecular Weight 22 kDa
GenBank Accession NumberBC009273
Gene Symbol GM2A
Gene ID (NCBI) 2760
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP17900
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

GM2A, also named as GM2-AP and SAP-3, is the large binding pocket which can accommodate several single chain phospholipids and fatty acids. GM2A also exhibits some calcium-independent phospholipase activity. It plays a key role in the degradation of ganglioside GM2 (GM2). GM2A stimulates only the breakdown of ganglioside GM2 and glycolipid GA2 by beta-hexosaminidase A.

实验方案

Product Specific Protocols
FC protocol for CL Plus 488 GM2A antibody CL488-66080Download protocol
Standard Protocols
Click here to view our Standard Protocols