验证数据展示
经过测试的应用
| Positive IF/ICC detected in | U-87 MG cells |
For other applications, we recommend the unconjugated version of this antibody, 60062-1-Ig
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL488-60062 targets GMF Beta in IF/ICC applications and shows reactivity with human, pig samples.
| 经测试应用 | IF/ICC Application Description |
| 经测试反应性 | human, pig |
| 免疫原 | GMF Beta fusion protein Ag1104 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | glia maturation factor, beta |
| 别名 | Glia maturation factor beta, glia maturation factor, beta, GMF, GMF beta, GMFB |
| 计算分子量 | 17 kDa |
| 观测分子量 | 17 kDa |
| GenBank蛋白编号 | BC005359 |
| 基因名称 | GMFB |
| Gene ID (NCBI) | 2764 |
| RRID | AB_3084134 |
| 偶联类型 | CoraLite® Plus 488 Fluorescent Dye |
| 最大激发/发射波长 | 493 nm / 522 nm |
| 形式 | Liquid |
| 纯化方式 | Protein G purification |
| UNIPROT ID | P60983 |
| 储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3 |
| 储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
GMFB, Glia maturation factor beta, is a nerve growth factor implicated in nervous system development. It was reported that GMF-beta promotes the phenotypic expression of glia and neurons and inhibits the proliferation of their respective tumors in culture cells. GMF-beta protein is specific to the brain where it is expressed in glial cells (mainly astrocytes) and insome neurons. Higher levels of GMFB were found in the central nervous system, except for the spinal cord, and in thymus and colon.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 GMF Beta antibody CL488-60062 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |