Geminin Recombinant antibody

Geminin Recombinant Antibody for IF/ICC, ELISA
Cat No. 84199-1-RR

产品说明书

CloneNo. 240835E6

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

IF/ICC, ELISA

GMNN, MGORS6, Gem, 240835E6

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -


经过测试的应用

Positive IF/ICC detected inHepG2 cells

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84199-1-RR targets Geminin in IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 IF/ICC, ELISA Application Description
经测试反应性 human
免疫原 Geminin fusion protein Ag1076 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 geminin, DNA replication inhibitor
别名 GMNN, MGORS6, Gem, 240835E6
计算分子量 24 kDa
GenBank蛋白编号BC005185
基因名称 GMNN
Gene ID (NCBI) 51053
RRIDAB_3671754
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDO75496
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

GMNN, also designated geminin, contains 212 amino acids and has a destruction box sequence (RRTLKVIQP). GMNN participates in ininhibiting DNA replication by preventing the incorporation of MCM complex into pre-replication complex (pre-RC). It is degraded during the metaphase-anaphase transition of cell cycle's mitotic phase, which permits replication in the succeeding cell cycle. GMNN has a broad sedimentation profile ranging from about 25 kDa to 90 kDa, with a major peak at 30 kDa. Scanning of the signals shows that discrete peaks corresponding to apparent mass of 42.5 kDa and 66 kDa are present. (PMID: 15313623)

实验方案

Product Specific Protocols
IF protocol for Geminin antibody 84199-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
Loading...