验证数据展示
经过测试的应用
Positive IF/ICC detected in | HepG2 cells |
推荐稀释比
应用 | 推荐稀释比 |
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Immunofluorescence (IF)/ICC | IF/ICC : 1:125-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
84199-1-RR targets Geminin in IF/ICC, ELISA applications and shows reactivity with human samples.
经测试应用 | IF/ICC, ELISA Application Description |
经测试反应性 | human |
免疫原 | Geminin fusion protein Ag1076 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Recombinant |
产品类型 | Antibody |
全称 | geminin, DNA replication inhibitor |
别名 | GMNN, MGORS6, Gem, 240835E6 |
计算分子量 | 24 kDa |
GenBank蛋白编号 | BC005185 |
基因名称 | GMNN |
Gene ID (NCBI) | 51053 |
RRID | AB_3671754 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Protein A purfication |
UNIPROT ID | O75496 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
GMNN, also designated geminin, contains 212 amino acids and has a destruction box sequence (RRTLKVIQP). GMNN participates in ininhibiting DNA replication by preventing the incorporation of MCM complex into pre-replication complex (pre-RC). It is degraded during the metaphase-anaphase transition of cell cycle's mitotic phase, which permits replication in the succeeding cell cycle. GMNN has a broad sedimentation profile ranging from about 25 kDa to 90 kDa, with a major peak at 30 kDa. Scanning of the signals shows that discrete peaks corresponding to apparent mass of 42.5 kDa and 66 kDa are present. (PMID: 15313623)
实验方案
Product Specific Protocols | |
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IF protocol for Geminin antibody 84199-1-RR | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |