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GNA14 Polyclonal antibody

GNA14 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 13350-1-AP

Print datasheet

Synonyms

Guanine nucleotide-binding protein subunit alpha-14, G-protein subunit alpha-14, G alpha-14

验证数据展示

  • WB analysis of mouse lung using 13350-1-AP

    WB analysis of mouse lung using 13350-1-AP

    mouse lung tissue were subjected to SDS PAGE followed by western blot with 13350-1-AP (GNA14 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IHC staining of human kidney using 13350-1-AP

    IHC staining of human kidney using 13350-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 13350-1-AP (GNA14 Antibody) at dilution of 1:200 (under 10x lens).

  • IHC staining of human kidney using 13350-1-AP

    IHC staining of human kidney using 13350-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 13350-1-AP (GNA14 Antibody) at dilution of 1:200 (under 40x lens).

  • IF Staining of HeLa using 13350-1-AP

    IF Staining of HeLa using 13350-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using 13350-1-AP (GNA14 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inmouse lung tissue
Positive IHC detected inhuman kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells, HepG2 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

13350-1-AP targets GNA14 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Tested Reactivity human, mouse
Immunogen GNA14 fusion protein Ag3930 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name guanine nucleotide binding protein (G protein), alpha 14
Synonyms Guanine nucleotide-binding protein subunit alpha-14, G-protein subunit alpha-14, G alpha-14
Calculated Molecular Weight 355 aa, 42 kDa
Observed Molecular Weight 42 kDa
GenBank Accession NumberBC027886
Gene Symbol GNA14
Gene ID (NCBI) 9630
RRIDAB_2877942
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO95837
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for GNA14 antibody 13350-1-APDownload protocol
IHC protocol for GNA14 antibody 13350-1-APDownload protocol
IF protocol for GNA14 antibody 13350-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols