GNAO1 Monoclonal antibody

GNAO1 Monoclonal Antibody for WB, IHC, IF-P, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Mouse, Rat, Rabbit, Pig, Chicken, Human

Applications

WB, IHC, IF-P, ELISA

Conjugate

Unconjugated

CloneNo.

1B5G2

Cat no : 68027-1-Ig

Print datasheet

Synonyms

DKFZp686O0962, G ALPHA o, GNAO, GNAO1



经过测试的应用

Positive WB detected inpig brain tissue, rabbit brain tissue, rat brain tissue, mouse brain tissue, chicken brain tissue, rat cerebellum tissue
Positive IHC detected inhuman gliomas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inrat cerebellum tissue

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
Immunofluorescence (IF)-PIF-P : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68027-1-Ig targets GNAO1 in WB, IHC, IF-P, ELISA applications and shows reactivity with Mouse, Rat, Rabbit, Pig, Chicken, Human samples.

Tested Applications WB, IHC, IF-P, ELISA Application Description
Tested Reactivity Mouse, Rat, Rabbit, Pig, Chicken, Human
Immunogen GNAO1 fusion protein Ag32045 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name guanine nucleotide binding protein (G protein), alpha activating activity polypeptide O
Synonyms DKFZp686O0962, G ALPHA o, GNAO, GNAO1
Calculated Molecular Weight 302 aa, 35 kDa
Observed Molecular Weight40 kDa
GenBank Accession NumberBC030027
Gene Symbol GNAO1
Gene ID (NCBI) 2775
RRIDAB_2918770
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP09471
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

GNAO1 gene encodes the α subunit of heterotrimeric guanine nucleotide-binding protein (Gi protein), a membrane protein widely expressed in the central nervous system involved in signal transduction (PMID: 30642806). GNAO1 belongs to the G-alpha family and G(i/o/t/z) subfamily. GNAO1 is highly expressed in brain and modulate neuronal excitability (PMID: 30838255). GNAO1 has 2 isoforms with the molecular mass of 40 kDa.

实验方案

Product Specific Protocols
WB protocol for GNAO1 antibody 68027-1-IgDownload protocol
IHC protocol for GNAO1 antibody 68027-1-IgDownload protocol
IF protocol for GNAO1 antibody 68027-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols