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GNAO1 Monoclonal antibody, PBS Only

GNAO1 Monoclonal Antibody for WB, IHC, IF-P, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Mouse, Rat, Rabbit, Pig, Chicken, Human

Applications

WB, IHC, IF-P, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1B5G2

Cat no : 68027-1-PBS

Print datasheet

Synonyms

DKFZp686O0962, G ALPHA o, GNAO, GNAO1

验证数据展示

  • WB analysis using 68027-1-Ig (same clone as 68027-1-PBS)

    WB analysis using 68027-1-Ig (same clone as 68027-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68027-1-Ig (GNAO1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with Lamin B1 Monoclonal antibody (66095-1-Ig) as loading control. This data was developed using the same antibody clone with 68027-1-PBS in a different storage buffer formulation.

  • IHC staining of human gliomas using 68027-1-Ig (same clone as 68027-1-PBS)

    IHC staining of human gliomas using 68027-1-Ig (same clone as 68027-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 68027-1-Ig (GNAO1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68027-1-PBS in a different storage buffer formulation.

  • IF Staining of rat cerebellum using 68027-1-Ig (same clone as 68027-1-PBS)

    IF Staining of rat cerebellum using 68027-1-Ig (same clone as 68027-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed rat cerebellum tissue using GNAO1 antibody (68027-1-Ig, Clone: 1B5G2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68027-1-PBS in a different storage buffer formulation.

产品信息

68027-1-PBS targets GNAO1 in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with Mouse, Rat, Rabbit, Pig, Chicken, Human samples.

Tested Applications WB, IHC, IF-P, Indirect ELISA Application Description
Tested Reactivity Mouse, Rat, Rabbit, Pig, Chicken, Human
Immunogen GNAO1 fusion protein Ag32045 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name guanine nucleotide binding protein (G protein), alpha activating activity polypeptide O
Synonyms DKFZp686O0962, G ALPHA o, GNAO, GNAO1
Calculated Molecular Weight 302 aa, 35 kDa
Observed Molecular Weight40 kDa
GenBank Accession NumberBC030027
Gene Symbol GNAO1
Gene ID (NCBI) 2775
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP09471
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

GNAO1 gene encodes the α subunit of heterotrimeric guanine nucleotide-binding protein (Gi protein), a membrane protein widely expressed in the central nervous system involved in signal transduction (PMID: 30642806). GNAO1 belongs to the G-alpha family and G(i/o/t/z) subfamily. GNAO1 is highly expressed in brain and modulate neuronal excitability (PMID: 30838255). GNAO1 has 2 isoforms with the molecular mass of 40 kDa.