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验证数据展示

  • WB analysis using 10081-1-AP (same clone as 10081-1-PBS)

    WB analysis using 10081-1-AP (same clone as 10081-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 10081-1-AP (GNB3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10081-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver cancer using 10081-1-AP (same clone as 10081-1-PBS)

    IHC staining of human liver cancer using 10081-1-AP (same clone as 10081-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 10081-1-AP (GNB3 Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 10081-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver cancer using 10081-1-AP (same clone as 10081-1-PBS)

    IHC staining of human liver cancer using 10081-1-AP (same clone as 10081-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 10081-1-AP (GNB3 Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 10081-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 10081-1-AP (same clone as 10081-1-PBS)

    IF Staining of HepG2 using 10081-1-AP (same clone as 10081-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using GNB3 antibody (10081-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). This data was developed using the same antibody clone with 10081-1-PBS in a different storage buffer formulation.

GNB3 Polyclonal antibody, PBS Only

GNB3 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 10081-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-3

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

10081-1-PBS targets GNB3 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag0127

Product name: Recombinant human GNB3 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-270 aa of BC000115

Sequence: MGEMEQLRQEAEQLKKQIADARKACADVTLAELVSGLEVVGRVQMRTRRTLRGHLAKIYAMHWATDSKLLVSASQDGKLIVWDSYTTNKVHAIPLRSSWVMTCAYAPSGNFVACGGLDNMCSIYNLKSREGNVKVSRELSAHTGYLSCCRFLDDNNIVTSSGDTTCALWDIETGQQKTVFVGHTGDCMSLAVSPDFNLFISGACDASAKLWDVREGTCRQTFTGHESDINAICFFPNGEAICTGSDDASCRLFDLRADQELICFSHESII

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 guanine nucleotide binding protein (G protein), beta polypeptide 3
别名 Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-3
计算分子量 37 kDa
观测分子量 35-37 kDa
GenBank蛋白编号BC000115
基因名称 GNB3
Gene ID (NCBI) 2784
RRIDAB_2263264
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP16520
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Guanine nucleotide-binding proteins (g proteins) are involved as a modulator or transducer in various transmembrane signaling systems, by integrating signals between receptors and effector proteins. G proteins are composed of an alpha, a beta, and a gamma subunit. This gene encodes a 34 kd beta subunit, being expressed in all tissues. Beta subunits are important regulators of alpha subunits, as well as of certain signal transduction receptors and effectors.

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