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GNB3 Monoclonal antibody

GNB3 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

2B6E1

Cat No : 67497-1-Ig

Print datasheet

Synonyms

Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-3, 2B6E1

验证数据展示

  • WB analysis using 67497-1-Ig

    WB analysis using 67497-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67497-1-Ig (GNB3 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver cancer using 67497-1-Ig

    IHC staining of human liver cancer using 67497-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67497-1-Ig (GNB3 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human liver cancer using 67497-1-Ig

    IHC staining of human liver cancer using 67497-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67497-1-Ig (GNB3 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HepG2 using 67497-1-Ig

    IF Staining of HepG2 using 67497-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using GNB3 antibody (67497-1-Ig, Clone: 2B6E1 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

经过测试的应用

Positive WB detected inhTERT-RPE1 cells, HepG2 cells, L02 cells, pig brain tissue, rat retina tissue, Raji cells, Ramos cells, Daudi cells, RKO cells, Karpas-422 cells, IMR-32 cells, Rat brain cells, Mouse brain cells, rat brain tissue, mouse brain tissue
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67497-1-Ig targets GNB3 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat, pig samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Tested Reactivity human, mouse, rat, pig
Immunogen GNB3 fusion protein Ag7050 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name guanine nucleotide binding protein (G protein), beta polypeptide 3
Synonyms Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-3, 2B6E1
Calculated Molecular Weight 37 kDa
Observed Molecular Weight 35-37 kDa
GenBank Accession NumberBC002454
Gene Symbol GNB3
Gene ID (NCBI) 2784
RRIDAB_2882721
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP16520
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Guanine nucleotide-binding proteins (g proteins) are involved as a modulator or transducer in various transmembrane signaling systems, by integrating signals between receptors and effector proteins. G proteins are composed of an alpha, a beta, and a gamma subunit. This gene encodes a 34 kd beta subunit, being expressed in all tissues. Beta subunits are important regulators of alpha subunits, as well as of certain signal transduction receptors and effectors.

实验方案

Product Specific Protocols
WB protocol for GNB3 antibody 67497-1-IgDownload protocol
IHC protocol for GNB3 antibody 67497-1-IgDownload protocol
IF protocol for GNB3 antibody 67497-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols