验证数据展示
经过测试的应用
Positive WB detected in | hTERT-RPE1 cells, HepG2 cells, L02 cells, pig brain tissue, rat retina tissue, Raji cells, Ramos cells, Daudi cells, RKO cells, Karpas-422 cells, IMR-32 cells, Rat brain cells, Mouse brain cells, rat brain tissue, mouse brain tissue |
Positive IHC detected in | human liver cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | HepG2 cells |
推荐稀释比
应用 | 推荐稀释比 |
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Western Blot (WB) | WB : 1:5000-1:50000 |
Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
67497-1-Ig targets GNB3 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat, pig samples.
经测试应用 | WB, IHC, IF/ICC, ELISA Application Description |
经测试反应性 | human, mouse, rat, pig |
免疫原 | GNB3 fusion protein Ag7050 种属同源性预测 |
宿主/亚型 | Mouse / IgG1 |
抗体类别 | Monoclonal |
产品类型 | Antibody |
全称 | guanine nucleotide binding protein (G protein), beta polypeptide 3 |
别名 | Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-3, 2B6E1 |
计算分子量 | 37 kDa |
观测分子量 | 35-37 kDa |
GenBank蛋白编号 | BC002454 |
基因名称 | GNB3 |
Gene ID (NCBI) | 2784 |
RRID | AB_2882721 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Protein G purification |
UNIPROT ID | P16520 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Guanine nucleotide-binding proteins (g proteins) are involved as a modulator or transducer in various transmembrane signaling systems, by integrating signals between receptors and effector proteins. G proteins are composed of an alpha, a beta, and a gamma subunit. This gene encodes a 34 kd beta subunit, being expressed in all tissues. Beta subunits are important regulators of alpha subunits, as well as of certain signal transduction receptors and effectors.
实验方案
Product Specific Protocols | |
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WB protocol for GNB3 antibody 67497-1-Ig | Download protocol |
IHC protocol for GNB3 antibody 67497-1-Ig | Download protocol |
IF protocol for GNB3 antibody 67497-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |