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GNB3 Monoclonal antibody, PBS Only

GNB3 Monoclonal Antibody for WB,Indirect ELISA,IHC,IF

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat, Pig

Applications

WB,Indirect ELISA,IHC,IF

Conjugate

Unconjugated

CloneNo.

2B6E1

Cat No : 67497-1-PBS

Print datasheet

Synonyms

GNB3, Transducin beta chain 3

验证数据展示

  • WB analysis using 67497-1-Ig (same clone as 67497-1-PBS)

    WB analysis using 67497-1-Ig (same clone as 67497-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67497-1-Ig (GNB3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67497-1-PBS in a different storage buffer formulation.

  • WB analysis of L02 using 67497-1-Ig (same clone as 67497-1-PBS)

    WB analysis of L02 using 67497-1-Ig (same clone as 67497-1-PBS)

    L02 cells were subjected to SDS PAGE followed by western blot with 67497-1-Ig (GNB3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67497-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver cancer using 67497-1-Ig (same clone as 67497-1-PBS)

    IHC staining of human liver cancer using 67497-1-Ig (same clone as 67497-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67497-1-Ig (GNB3 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67497-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver cancer using 67497-1-Ig (same clone as 67497-1-PBS)

    IHC staining of human liver cancer using 67497-1-Ig (same clone as 67497-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67497-1-Ig (GNB3 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67497-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 67497-1-Ig (same clone as 67497-1-PBS)

    IF Staining of HepG2 using 67497-1-Ig (same clone as 67497-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using GNB3 antibody (67497-1-Ig, Clone: 2B6E1 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67497-1-PBS in a different storage buffer formulation.

产品信息

67497-1-PBS targets GNB3 in WB, IF, IHC, Indirect ELISA applications and shows reactivity with Human, Mouse, Rat, Pig samples.

Tested Applications WB,Indirect ELISA,IHC,IF Application Description
Tested Reactivity Human, Mouse, Rat, Pig
Immunogen GNB3 fusion protein Ag7050 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name guanine nucleotide binding protein (G protein), beta polypeptide 3
Synonyms GNB3, Transducin beta chain 3
Calculated Molecular Weight 37 kDa
Observed Molecular Weight 35-37 kDa
GenBank Accession NumberBC002454
Gene Symbol GNB3
Gene ID (NCBI) 2784
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP16520
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

背景介绍

Guanine nucleotide-binding proteins (g proteins) are involved as a modulator or transducer in various transmembrane signaling systems, by integrating signals between receptors and effector proteins. G proteins are composed of an alpha, a beta, and a gamma subunit. This gene encodes a 34 kd beta subunit, being expressed in all tissues. Beta subunits are important regulators of alpha subunits, as well as of certain signal transduction receptors and effectors.