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GNMT Polyclonal antibody, PBS Only

GNMT Polyclonal Antibody for WB, IHC, IP, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, Indirect ELISA

Conjugate

Unconjugated

Cat no : 18790-1-PBS

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Synonyms

验证数据展示

  • WB analysis of rat liver using 18790-1-AP (same clone as 18790-1-PBS)

    WB analysis of rat liver using 18790-1-AP (same clone as 18790-1-PBS)

    rat liver tissue were subjected to SDS PAGE followed by western blot with 18790-1-AP (GNMT antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18790-1-PBS in a different storage buffer formulation.

  • WB analysis using 18790-1-AP (same clone as 18790-1-PBS)

    WB analysis using 18790-1-AP (same clone as 18790-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 18790-1-AP (GNMT antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18790-1-PBS in a different storage buffer formulation.

  • IHC staining of human prostate hyperplasia using 18790-1-AP (same clone as 18790-1-PBS)

    IHC staining of human prostate hyperplasia using 18790-1-AP (same clone as 18790-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate hyperplasia tissue slide using 18790-1-AP (GNMT antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 18790-1-PBS in a different storage buffer formulation.

  • IP experiment of mouse liver using 18790-1-AP (same clone as 18790-1-PBS)

    IP experiment of mouse liver using 18790-1-AP (same clone as 18790-1-PBS)

    IP result of anti-GNMT (IP:18790-1-AP, 4ug; Detection:18790-1-AP 1:8000) with mouse liver tissue lysate 3040 ug. This data was developed using the same antibody clone with 18790-1-PBS in a different storage buffer formulation.

  • IP experiment of mouse liver using 18790-1-AP (same clone as 18790-1-PBS)

    IP experiment of mouse liver using 18790-1-AP (same clone as 18790-1-PBS)

    IP result of anti-GNMT (IP:18790-1-AP, 4ug; Detection:18790-1-AP 1:8000) with mouse liver tissue lysate 1840 ug. This data was developed using the same antibody clone with 18790-1-PBS in a different storage buffer formulation.

产品信息

18790-1-PBS targets GNMT in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IP, Indirect ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen GNMT fusion protein Ag4598 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name glycine N-methyltransferase
Synonyms
Calculated Molecular Weight 295 aa, 33 kDa
Observed Molecular Weight 33 kDa
GenBank Accession NumberBC032627
Gene Symbol GNMT
Gene ID (NCBI) 27232
RRIDAB_10597093
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ14749
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Glycine N-methyltransferase (GNMT, EC 2.1.1.20) was found originally as an enzyme regulating the ratio of SAM to S-adenosyl- homocysteine. GNMT is conservative among different animal species. Glycine-N methyltransferase (GNMT) is a potential tumor suppressor that is commonly inactivated in human hepatoma. GNMT is abundant in liver, but very low in HepG2 cells (PMID: 12566990).