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GNMT Monoclonal antibody, PBS Only

GNMT Monoclonal Antibody for WB, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Pig, Rat

Applications

WB, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1B5E3

Cat No : 67294-1-PBS

Print datasheet

Synonyms

glycine N methyltransferase, GNMT

验证数据展示

  • WB analysis using 67294-1-Ig (same clone as 67294-1-PBS)

    WB analysis using 67294-1-Ig (same clone as 67294-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67294-1-Ig (GNMT antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67294-1-PBS in a different storage buffer formulation.

  • IF Staining of A431 using 67294-1-Ig (same clone as 67294-1-PBS)

    IF Staining of A431 using 67294-1-Ig (same clone as 67294-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using GNMT antibody (67294-1-Ig, Clone: 1B5E3 ) at dilution of 1:800 and CoraLite®594-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67294-1-PBS in a different storage buffer formulation.

产品信息

67294-1-PBS targets GNMT in WB, IF/ICC, Indirect ELISA applications and shows reactivity with Human, Mouse, Pig, Rat samples.

Tested Applications WB, IF/ICC, Indirect ELISA Application Description
Tested Reactivity Human, Mouse, Pig, Rat
Immunogen GNMT fusion protein Ag4598 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name glycine N-methyltransferase
Synonyms glycine N methyltransferase, GNMT
Calculated Molecular Weight 295 aa, 33 kDa
Observed Molecular Weight 33 kDa
GenBank Accession NumberBC032627
Gene Symbol GNMT
Gene ID (NCBI) 27232
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ14749
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Glycine N-methyltransferase (GNMT, EC 2.1.1.20) was found originally as an enzyme regulating the ratio of SAM to S-adenosyl- homocysteine. GNMT is conservative among different animal species. Glycine-N methyltransferase (GNMT) is a potential tumor suppressor that is commonly inactivated in human hepatoma. GNMT is abundant in liver, but very low in HepG2 cells (PMID: 12566990).