GNMT Monoclonal antibody
GNMT Monoclonal Antibody for WB, IF/ICC, ELISA
Host / Isotype
Mouse / IgG1
Reactivity
Human, Mouse, Pig, Rat and More (1)
Applications
WB, IF/ICC, ELISA and More (1)
Conjugate
Unconjugated
CloneNo.
1B5E3
验证数据展示
经过测试的应用
| Positive WB detected in | rat liver tissue, pig liver tissue, mouse liver tissue, rat pancreas tissue, mouse pancreas tissue |
| Positive IF/ICC detected in | A431 cells |
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:400-1:1600 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 1 publications below |
| IHC | See 1 publications below |
产品信息
67294-1-Ig targets GNMT in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, Mouse, Pig, Rat samples.
| Tested Applications | WB, IF/ICC, ELISA Application Description |
| Cited Applications | WB, IHC |
| Tested Reactivity | Human, Mouse, Pig, Rat |
| Cited Reactivity | human |
| Immunogen | GNMT fusion protein Ag4598 种属同源性预测 |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | glycine N-methyltransferase |
| Synonyms | glycine N methyltransferase, GNMT |
| Calculated Molecular Weight | 295 aa, 33 kDa |
| Observed Molecular Weight | 33 kDa |
| GenBank Accession Number | BC032627 |
| Gene Symbol | GNMT |
| Gene ID (NCBI) | 27232 |
| RRID | AB_2882559 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q14749 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Glycine N-methyltransferase (GNMT, EC 2.1.1.20) was found originally as an enzyme regulating the ratio of SAM to S-adenosyl- homocysteine. GNMT is conservative among different animal species. Glycine-N methyltransferase (GNMT) is a potential tumor suppressor that is commonly inactivated in human hepatoma. GNMT is abundant in liver, but very low in HepG2 cells (PMID: 12566990).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for GNMT antibody 67294-1-Ig | Download protocol |
| IF protocol for GNMT antibody 67294-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Front Oncol Mining of RNA Methylation-Related Genes and Elucidation of Their Molecular Biology in Gallbladder Carcinoma. | ||
Cell Biochem Biophys Brucine Suppresses Malignant Progression of Prostate Cancer by Decreasing Sarcosine Accumulation via Downregulation of GNMT in the Glycine/sarcosine Metabolic Pathway |
