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验证数据展示

  • WB analysis using 67294-1-Ig

    WB analysis using 67294-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67294-1-Ig (GNMT antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IF Staining of A431 using 67294-1-Ig

    IF Staining of A431 using 67294-1-Ig

    Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using GNMT antibody (67294-1-Ig, Clone: 1B5E3 ) at dilution of 1:800 and CoraLite®594-Conjugated Goat Anti-Mouse IgG(H+L).

GNMT Monoclonal antibody

GNMT Monoclonal Antibody for WB, IF/ICC, ELISA
Cat No. 67294-1-Ig

Print datasheet

CloneNo. 1B5E3
Publications(2)

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Pig, Rat and More (1)

Applications

WB, IF/ICC, ELISA and More (1)

Synonyms

glycine N methyltransferase, GNMT

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size: 

-/ -

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经过测试的应用

Positive WB detected inrat liver tissue, pig liver tissue, mouse liver tissue, rat pancreas tissue, mouse pancreas tissue
Positive IF/ICC detected inA431 cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67294-1-Ig targets GNMT in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, Mouse, Pig, Rat samples.

Tested Applications WB, IF/ICC, ELISA Application Description
Cited ApplicationsWB, IHC
Tested Reactivity Human, Mouse, Pig, Rat
Cited Reactivityhuman
Immunogen GNMT fusion protein Ag4598 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name glycine N-methyltransferase
Synonyms glycine N methyltransferase, GNMT
Calculated Molecular Weight 295 aa, 33 kDa
Observed Molecular Weight 33 kDa
GenBank Accession NumberBC032627
Gene Symbol GNMT
Gene ID (NCBI) 27232
RRIDAB_2882559
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ14749
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Glycine N-methyltransferase (GNMT, EC 2.1.1.20) was found originally as an enzyme regulating the ratio of SAM to S-adenosyl- homocysteine. GNMT is conservative among different animal species. Glycine-N methyltransferase (GNMT) is a potential tumor suppressor that is commonly inactivated in human hepatoma. GNMT is abundant in liver, but very low in HepG2 cells (PMID: 12566990).

实验方案

Product Specific Protocols
WB protocol for GNMT antibody 67294-1-IgDownload protocol
IF protocol for GNMT antibody 67294-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIHC

Front Oncol

Mining of RNA Methylation-Related Genes and Elucidation of Their Molecular Biology in Gallbladder Carcinoma.

Authors - Changhong Yang
View Article
humanWB

Cell Biochem Biophys

Brucine Suppresses Malignant Progression of Prostate Cancer by Decreasing Sarcosine Accumulation via Downregulation of GNMT in the Glycine/sarcosine Metabolic Pathway

Authors - Long Miao
View Article