GNMT Monoclonal antibody

GNMT Monoclonal Antibody for WB, IF/ICC, ELISA
Cat No. 67294-1-Ig

产品说明书

CloneNo. 1B5E3

宿主/亚型

Mouse / IgG1

种属反应性

Human, Mouse, Pig, Rat and More (1)

应用

WB, IF/ICC, ELISA and More (1)

glycine N methyltransferase, GNMT

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inrat liver tissue, pig liver tissue, mouse liver tissue, rat pancreas tissue, mouse pancreas tissue
Positive IF/ICC detected inA431 cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67294-1-Ig targets GNMT in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, Mouse, Pig, Rat samples.

经测试应用 WB, IF/ICC, ELISA Application Description
文献引用应用WB, IHC
经测试反应性 Human, Mouse, Pig, Rat
文献引用反应性human
免疫原 GNMT fusion protein Ag4598 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 glycine N-methyltransferase
别名 glycine N methyltransferase, GNMT
计算分子量 295 aa, 33 kDa
观测分子量 33 kDa
GenBank蛋白编号BC032627
基因名称 GNMT
Gene ID (NCBI) 27232
RRIDAB_2882559
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ14749
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Glycine N-methyltransferase (GNMT, EC 2.1.1.20) was found originally as an enzyme regulating the ratio of SAM to S-adenosyl- homocysteine. GNMT is conservative among different animal species. Glycine-N methyltransferase (GNMT) is a potential tumor suppressor that is commonly inactivated in human hepatoma. GNMT is abundant in liver, but very low in HepG2 cells (PMID: 12566990).

实验方案

Product Specific Protocols
WB protocol for GNMT antibody 67294-1-IgDownload protocol
IF protocol for GNMT antibody 67294-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIHC

Front Oncol

Mining of RNA Methylation-Related Genes and Elucidation of Their Molecular Biology in Gallbladder Carcinoma.

Authors - Changhong Yang
humanWB

Cell Biochem Biophys

Brucine Suppresses Malignant Progression of Prostate Cancer by Decreasing Sarcosine Accumulation via Downregulation of GNMT in the Glycine/sarcosine Metabolic Pathway

Authors - Long Miao
Loading...