验证数据展示
经过测试的应用
Positive WB detected in | rat liver tissue, pig liver tissue, mouse liver tissue, rat pancreas tissue, mouse pancreas tissue |
Positive IF/ICC detected in | A431 cells |
推荐稀释比
应用 | 推荐稀释比 |
---|---|
Western Blot (WB) | WB : 1:5000-1:50000 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:400-1:1600 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
发表文章中的应用
WB | See 1 publications below |
IHC | See 1 publications below |
产品信息
67294-1-Ig targets GNMT in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, Mouse, Pig, Rat samples.
经测试应用 | WB, IF/ICC, ELISA Application Description |
文献引用应用 | WB, IHC |
经测试反应性 | Human, Mouse, Pig, Rat |
文献引用反应性 | human |
免疫原 | GNMT fusion protein Ag4598 种属同源性预测 |
宿主/亚型 | Mouse / IgG1 |
抗体类别 | Monoclonal |
产品类型 | Antibody |
全称 | glycine N-methyltransferase |
别名 | glycine N methyltransferase, GNMT |
计算分子量 | 295 aa, 33 kDa |
观测分子量 | 33 kDa |
GenBank蛋白编号 | BC032627 |
基因名称 | GNMT |
Gene ID (NCBI) | 27232 |
RRID | AB_2882559 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Protein A purification |
UNIPROT ID | Q14749 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Glycine N-methyltransferase (GNMT, EC 2.1.1.20) was found originally as an enzyme regulating the ratio of SAM to S-adenosyl- homocysteine. GNMT is conservative among different animal species. Glycine-N methyltransferase (GNMT) is a potential tumor suppressor that is commonly inactivated in human hepatoma. GNMT is abundant in liver, but very low in HepG2 cells (PMID: 12566990).
实验方案
Product Specific Protocols | |
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WB protocol for GNMT antibody 67294-1-Ig | Download protocol |
IF protocol for GNMT antibody 67294-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Front Oncol Mining of RNA Methylation-Related Genes and Elucidation of Their Molecular Biology in Gallbladder Carcinoma. | ||
Cell Biochem Biophys Brucine Suppresses Malignant Progression of Prostate Cancer by Decreasing Sarcosine Accumulation via Downregulation of GNMT in the Glycine/sarcosine Metabolic Pathway |