验证数据展示
经过测试的应用
| Positive IF/ICC detected in | A431 cells |
For other applications, we recommend the unconjugated version of this antibody, 67294-1-Ig
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL594-67294 targets GNMT in IF/ICC applications and shows reactivity with Human, Mouse, Pig, Rat samples.
| 经测试应用 | IF/ICC Application Description |
| 经测试反应性 | Human, Mouse, Pig, Rat |
| 免疫原 | GNMT fusion protein Ag4598 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | glycine N-methyltransferase |
| 别名 | glycine N methyltransferase, GNMT |
| 计算分子量 | 295 aa, 33 kDa |
| 观测分子量 | 33 kDa |
| GenBank蛋白编号 | BC032627 |
| 基因名称 | GNMT |
| Gene ID (NCBI) | 27232 |
| RRID | AB_2934762 |
| 偶联类型 | CoraLite®594 Fluorescent Dye |
| 最大激发/发射波长 | 588 nm / 604 nm |
| 形式 | Liquid |
| 纯化方式 | Protein G purification |
| UNIPROT ID | Q14749 |
| 储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3 |
| 储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Glycine N-methyltransferase (GNMT, EC 2.1.1.20) was found originally as an enzyme regulating the ratio of SAM to S-adenosyl- homocysteine. GNMT is conservative among different animal species. Glycine-N methyltransferase (GNMT) is a potential tumor suppressor that is commonly inactivated in human hepatoma. GNMT is abundant in liver, but very low in HepG2 cells (PMID: 12566990).
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL594 GNMT antibody CL594-67294 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |