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验证数据展示

  • WB analysis of HeLa using 66453-1-Ig

    WB analysis of HeLa using 66453-1-Ig

    HeLa cells were subjected to SDS PAGE followed by western blot with 66453-1-Ig (GNPAT antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver cancer using 66453-1-Ig

    IHC staining of human liver cancer using 66453-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66453-1-Ig (GNPAT antibody) at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human liver cancer using 66453-1-Ig

    IHC staining of human liver cancer using 66453-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66453-1-Ig (GNPAT antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

GNPAT Monoclonal antibody

GNPAT Monoclonal Antibody for WB, IHC, ELISA
Cat No. 66453-1-Ig

产品说明书

CloneNo. 2E5C10

宿主/亚型

Mouse / IgG2b

种属反应性

human

应用

WB, IHC, ELISA

别名

DAP AT, DAPAT, DHAP AT, DHAPAT, GNPAT

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, COLO 320 cells, HepG2 cells
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66453-1-Ig targets GNPAT in WB, IHC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human
免疫原 GNPAT fusion protein Ag6740 种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 glyceronephosphate O-acyltransferase
别名 DAP AT, DAPAT, DHAP AT, DHAPAT, GNPAT
计算分子量 77 kDa
观测分子量 65-69 kDa
GenBank蛋白编号BC000450
基因名称 GNPAT
Gene ID (NCBI) 8443
RRIDAB_2881822
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDO15228
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

GNPAT, also named as DAPAT and DHAPAT, belongs to the GPAT/DAPAT family. It is a key enzyme in the biosynthesis of ether phospholipids. GNPAT is localized exclusively within peroxisomes. Full GNPAT activity depends not only on the presence of AGPS, but also on the integrity of substrate channeling from GNPAT to AGPS. (PMID: 21990100) This antibody recognize the 65-69 kDa GNPAT protein.

实验方案

Product Specific Protocols
WB protocol for GNPAT antibody 66453-1-IgDownload protocol
IHC protocol for GNPAT antibody 66453-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols