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GNPAT Monoclonal antibody, PBS Only

GNPAT Monoclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2E5C10

Cat no : 66453-1-PBS

Print datasheet

Synonyms

DAP AT, DAPAT, DHAP AT, DHAPAT, GNPAT

验证数据展示

  • WB analysis of HeLa using 66453-1-Ig (same clone as 66453-1-PBS)

    WB analysis of HeLa using 66453-1-Ig (same clone as 66453-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 66453-1-Ig (GNPAT antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66453-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver cancer using 66453-1-Ig (same clone as 66453-1-PBS)

    IHC staining of human liver cancer using 66453-1-Ig (same clone as 66453-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66453-1-Ig (GNPAT antibody) at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66453-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver cancer using 66453-1-Ig (same clone as 66453-1-PBS)

    IHC staining of human liver cancer using 66453-1-Ig (same clone as 66453-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66453-1-Ig (GNPAT antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66453-1-PBS in a different storage buffer formulation.

产品信息

66453-1-PBS targets GNPAT in WB, IHC, Indirect ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, Indirect ELISA Application Description
Tested Reactivity human
Immunogen GNPAT fusion protein Ag6740 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name glyceronephosphate O-acyltransferase
Synonyms DAP AT, DAPAT, DHAP AT, DHAPAT, GNPAT
Calculated Molecular Weight 77 kDa
Observed Molecular Weight 65-69 kDa
GenBank Accession NumberBC000450
Gene Symbol GNPAT
Gene ID (NCBI) 8443
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDO15228
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

GNPAT, also named as DAPAT and DHAPAT, belongs to the GPAT/DAPAT family. It is a key enzyme in the biosynthesis of ether phospholipids. GNPAT is localized exclusively within peroxisomes. Full GNPAT activity depends not only on the presence of AGPS, but also on the integrity of substrate channeling from GNPAT to AGPS. (PMID: 21990100) This antibody recognize the 65-69 kDa GNPAT protein.