验证数据展示
经过测试的应用
| Positive WB detected in | HEK-293 cells, SMMC-7721 cells, HeLa cells, HepG2 cells |
| Positive IF/ICC detected in | HeLa cells |
| Positive FC (Intra) detected in | HeLa cells |
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:16000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:500-1:2000 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
12640-1-AP targets golgin 97 in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IF/ICC, FC (Intra), ELISA Application Description |
| 文献引用应用 | WB, IHC, IF, IP |
| 经测试反应性 | human |
| 文献引用反应性 | human, mouse, monkey |
| 免疫原 | golgin 97 fusion protein Ag3335 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | golgi autoantigen, golgin subfamily a, 1 |
| 别名 | GOLGA1, Golgin-97, Golgin subfamily A member 1 |
| 计算分子量 | 767 aa, 88 kDa |
| 观测分子量 | 97 kDa |
| GenBank蛋白编号 | BC032853 |
| 基因名称 | golgin 97 |
| Gene ID (NCBI) | 2800 |
| RRID | AB_2115315 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q92805 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Golgin subfamily A member 1 is a protein that in humans is encoded by the GOLGA1 gene. GOLGA1 has been shown to interact with ARL1. GOLGA1 is associated with Sjogren's syndrome. The MW of this protein is 97 kDa, and Catalog#12640-1-AP specially recognises the 97 kDa protein. IF result shows strong Gogli staining.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for golgin 97 antibody 12640-1-AP | Download protocol |
| IF protocol for golgin 97 antibody 12640-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nat Commun Depletion of mitochondrial methionine adenosyltransferase α1 triggers mitochondrial dysfunction in alcohol-associated liver disease. | ||
Redox Biol Selenoprotein K contributes to CD36 subcellular trafficking in hepatocytes by accelerating nascent COPII vesicle formation and aggravates hepatic steatosis | ||
Dev Cell GRASP55 Senses Glucose Deprivation through O-GlcNAcylation to Promote Autophagosome-Lysosome Fusion. | ||
