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验证数据展示

  • IF Staining of HeLa using CL488-12640

    IF Staining of HeLa using CL488-12640

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite® Plus 488 golgin 97 antibody (CL488-12640) at dilution of 1:400, CL594-phalloidin (red).

  • FC experiment of HeLa using CL488-12640

    FC experiment of HeLa using CL488-12640

    1X10^6 HeLa cells were intracellularly stained with 0.8 ug CoraLite® Plus 488 Anti-Human golgin 97 (CL488-12640) (red), or 0.8 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

CoraLite® Plus 488-conjugated golgin 97 Polyclonal antibody

golgin 97 Polyclonal Antibody for IF/ICC, FC (Intra)
Cat No. CL488-12640

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

IF/ICC, FC (Intra)

别名

GOLGA1, Golgin-97, Golgin subfamily A member 1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inHeLa cells
For other applications, we recommend the unconjugated version of this antibody, 12640-1-AP

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-12640 targets golgin 97 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse samples.

经测试应用 IF/ICC, FC (Intra) Application Description
经测试反应性 human, mouse
免疫原 golgin 97 fusion protein Ag3335 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 golgi autoantigen, golgin subfamily a, 1
别名 GOLGA1, Golgin-97, Golgin subfamily A member 1
计算分子量 767 aa, 88 kDa
观测分子量 97 kDa
GenBank蛋白编号BC032853
基因名称 golgin 97
Gene ID (NCBI) 2800
RRIDAB_3083930
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ92805
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Golgin subfamily A member 1 is a protein that in humans is encoded by the GOLGA1 gene. GOLGA1 has been shown to interact with ARL1. GOLGA1 is associated with Sjogren's syndrome. The MW of this protein is 97 kDa. IF result shows strong Gogli staining.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 golgin 97 antibody CL488-12640Download protocol
FC protocol for CL Plus 488 golgin 97 antibody CL488-12640Download protocol
Standard Protocols
Click here to view our Standard Protocols