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golgin 97 Monoclonal antibody

golgin 97 Monoclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

2A5E4

Cat no : 68648-1-Ig

Print datasheet

Synonyms

GOLGA1, golgin 97, Golgin subfamily A member 1

验证数据展示

  • WB analysis using 68648-1-Ig

    WB analysis using 68648-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68648-1-Ig (golgin 97 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IF Staining of MCF-7 using 68648-1-Ig

    IF Staining of MCF-7 using 68648-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using golgin 97 antibody (68648-1-Ig, Clone: 2A5E4 ) at dilution of 1:500 and Multi-rAb CoraLite ® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM004).

  • IF Staining of HeLa using 68648-1-Ig

    IF Staining of HeLa using 68648-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using golgin 97 antibody (68648-1-Ig, Clone: 2A5E4 ) at dilution of 1:500 and Multi-rAb CoraLite ® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM004).

经过测试的应用

Positive WB detected inA549 cells, LNCaP cells, MCF-7 cells, HeLa cells
Positive IF/ICC detected inMCF-7 cells, HeLa cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunofluorescence (IF)/ICCIF/ICC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68648-1-Ig targets golgin 97 in WB, IF/ICC, ELISA applications and shows reactivity with Human samples.

Tested Applications WB, IF/ICC, ELISA Application Description
Tested Reactivity Human
Immunogen golgin 97 fusion protein Ag33790 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name golgi autoantigen, golgin subfamily a, 1
Synonyms GOLGA1, golgin 97, Golgin subfamily A member 1
Calculated Molecular Weight 767 aa, 88 kDa
Observed Molecular Weight 97 kDa
GenBank Accession NumberBC032853
Gene Symbol golgin 97
Gene ID (NCBI) 2800
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ92805
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Golgin subfamily A member 1 is a protein that in humans is encoded by the GOLGA1 gene. GOLGA1 has been shown to interact with ARL1. GOLGA1 is associated with Sjogren's syndrome. The MW of this protein is 97 kDa. IF result shows strong Gogli staining.

实验方案

Product Specific Protocols
WB protocol for golgin 97 antibody 68648-1-IgDownload protocol
IF protocol for golgin 97 antibody 68648-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols