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验证数据展示

  • WB analysis using 68648-1-Ig

    WB analysis using 68648-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68648-1-Ig (golgin 97 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IF Staining of MCF-7 using 68648-1-Ig

    IF Staining of MCF-7 using 68648-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using golgin 97 antibody (68648-1-Ig, Clone: 2A5E4 ) at dilution of 1:500 and Multi-rAb CoraLite ® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM004).

  • IF Staining of HeLa using 68648-1-Ig

    IF Staining of HeLa using 68648-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using golgin 97 antibody (68648-1-Ig, Clone: 2A5E4 ) at dilution of 1:500 and Multi-rAb CoraLite ® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM004).

golgin 97 Monoclonal antibody

golgin 97 Monoclonal Antibody for WB, IF/ICC, ELISA
Cat No. 68648-1-Ig

产品说明书

CloneNo. 2A5E4

宿主/亚型

Mouse / IgG2a

种属反应性

Human

应用

WB, IF/ICC, ELISA

别名

GOLGA1, golgin 97, Golgin subfamily A member 1

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inA549 cells, LNCaP cells, MCF-7 cells, HeLa cells
Positive IF/ICC detected inMCF-7 cells, HeLa cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
Immunofluorescence (IF)/ICCIF/ICC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68648-1-Ig targets golgin 97 in WB, IF/ICC, ELISA applications and shows reactivity with Human samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 Human
免疫原 golgin 97 fusion protein Ag33790 种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 golgi autoantigen, golgin subfamily a, 1
别名 GOLGA1, golgin 97, Golgin subfamily A member 1
计算分子量 767 aa, 88 kDa
观测分子量 97 kDa
GenBank蛋白编号BC032853
基因名称 golgin 97
Gene ID (NCBI) 2800
RRIDAB_3670391
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ92805
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Golgin subfamily A member 1 is a protein that in humans is encoded by the GOLGA1 gene. GOLGA1 has been shown to interact with ARL1. GOLGA1 is associated with Sjogren's syndrome. The MW of this protein is 97 kDa. IF result shows strong Gogli staining.

实验方案

Product Specific Protocols
WB protocol for golgin 97 antibody 68648-1-IgDownload protocol
IF protocol for golgin 97 antibody 68648-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols