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验证数据展示

  • WB analysis using 68648-1-Ig (same clone as 68648-1-PBS)

    WB analysis using 68648-1-Ig (same clone as 68648-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68648-1-Ig (golgin 97 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68648-1-PBS in a different storage buffer formulation.

  • IF Staining of MCF-7 using 68648-1-Ig (same clone as 68648-1-PBS)

    IF Staining of MCF-7 using 68648-1-Ig (same clone as 68648-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using golgin 97 antibody (68648-1-Ig, Clone: 2A5E4 ) at dilution of 1:0 and Multi-rAb CoraLite ® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM004). This data was developed using the same antibody clone with 68648-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 68648-1-Ig (same clone as 68648-1-PBS)

    IF Staining of HeLa using 68648-1-Ig (same clone as 68648-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using golgin 97 antibody (68648-1-Ig, Clone: 2A5E4 ) at dilution of 1:0 and Multi-rAb CoraLite ® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM004). This data was developed using the same antibody clone with 68648-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP50871-1

    Cytometric bead array standard curve of MP50871-1

    Cytometric bead array standard curve of MP50871-1, GOLGA1 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 68648-2-PBS. Detection antibody: 68648-1-PBS. Standard:Ag33790. Range: 0.195-100 ng/mL.

  • Cytometric bead array standard curve of MP50871-3

    Cytometric bead array standard curve of MP50871-3

    Cytometric bead array standard curve of MP50871-3, GOLGA1 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 68648-3-PBS. Detection antibody: 68648-1-PBS. Standard:Ag33790. Range: 1.563-100 ng/mL.

golgin 97 Monoclonal antibody, PBS Only (Detector)

golgin 97 Monoclonal Antibody for WB, IF/ICC, Cytometric bead array, Indirect ELISA
Cat No. 68648-1-PBS

产品说明书

CloneNo. 2A5E4

宿主/亚型

Mouse / IgG2a

种属反应性

human

应用

WB, IF/ICC, Cytometric bead array, Indirect ELISA

别名

GOLGA1, Golgin-97, Golgin subfamily A member 1, 2A5E4

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

68648-1-PBS targets golgin 97 as part of a matched antibody pair:

MP50871-1: 68648-2-PBS capture and 68648-1-PBS detection (validated in Cytometric bead array)

MP50871-3: 68648-3-PBS capture and 68648-1-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, IF/ICC, Cytometric bead array, Indirect ELISA Application Description
经测试反应性 human
免疫原 golgin 97 fusion protein Ag33790 种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 golgi autoantigen, golgin subfamily a, 1
别名 GOLGA1, Golgin-97, Golgin subfamily A member 1, 2A5E4
计算分子量 767 aa, 88 kDa
观测分子量 97 kDa
GenBank蛋白编号BC032853
基因名称 golgin 97
Gene ID (NCBI) 2800
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ92805
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Golgin subfamily A member 1 is a protein that in humans is encoded by the GOLGA1 gene. GOLGA1 has been shown to interact with ARL1. GOLGA1 is associated with Sjogren's syndrome. The MW of this protein is 97 kDa. IF result shows strong Gogli staining.