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golgin 97 Recombinant antibody, PBS Only

golgin 97 Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Conjugate

Unconjugated

CloneNo.

230523A5

Cat no : 83009-4-PBS

Print datasheet

Synonyms

GOLGA1, Golgin-97, Golgin subfamily A member 1, 230523A5

验证数据展示

  • WB analysis using 83009-4-RR (same clone as 83009-4-PBS)

    WB analysis using 83009-4-RR (same clone as 83009-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83009-4-RR (golgin 97 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83009-4-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 83009-4-RR (same clone as 83009-4-PBS)

    IF Staining of HepG2 using 83009-4-RR (same clone as 83009-4-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using golgin 97 antibody (83009-4-RR, Clone: 230523A5 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83009-4-PBS in a different storage buffer formulation.

  • FC experiment of HepG2 using 83009-4-RR (same clone as 83009-4-PBS)

    FC experiment of HepG2 using 83009-4-RR (same clone as 83009-4-PBS)

    1x10^6 HepG2 cells were intracellularly stained with 0.25 ug golgin 97 Recombinant antibody (83009-4-RR, Clone:230523A5) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Control Antibody (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83009-4-PBS in a different storage buffer formulation.

  • IHC staining of human ovary cancer using 83009-4-RR (same clone as 83009-4-PBS)

    IHC staining of human ovary cancer using 83009-4-RR (same clone as 83009-4-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 83009-4-RR (golgin 97 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83009-4-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83009-4-RR

    Affinity and Kinetic Characterization of 83009-4-RR

    Biolayer interferometry (BLl) kinetic assays of 83009-4-RR against Human golgin 97 were performed. The affinity constant is below 1 pM.

产品信息

83009-4-PBS targets golgin 97 in WB, IHC, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, IF/ICC, FC (Intra), Indirect ELISA Application Description
Tested Reactivity human
Immunogen golgin 97 fusion protein Ag3335 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name golgi autoantigen, golgin subfamily a, 1
Synonyms GOLGA1, Golgin-97, Golgin subfamily A member 1, 230523A5
Calculated Molecular Weight 767 aa, 88 kDa
Observed Molecular Weight 97 kDa
GenBank Accession NumberBC032853
Gene Symbol golgin 97
Gene ID (NCBI) 2800
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ92805
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Golgin subfamily A member 1 is a protein that in humans is encoded by the GOLGA1 gene. GOLGA1 has been shown to interact with ARL1. GOLGA1 is associated with Sjogren's syndrome. IF result shows strong Gogli staining.