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验证数据展示

  • WB analysis using 83009-4-RR

    WB analysis using 83009-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83009-4-RR (golgin 97 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IF Staining of HepG2 using 83009-4-RR

    IF Staining of HepG2 using 83009-4-RR

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using golgin 97 antibody (83009-4-RR, Clone: 230523A5 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • FC experiment of HepG2 using 83009-4-RR

    FC experiment of HepG2 using 83009-4-RR

    1x10^6 HepG2 cells were intracellularly stained with 0.25 ug golgin 97 Recombinant antibody (83009-4-RR, Clone:230523A5) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Control Antibody (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • IHC staining of human ovary cancer using 83009-4-RR

    IHC staining of human ovary cancer using 83009-4-RR

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 83009-4-RR (golgin 97 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Affinity and Kinetic Characterization of 83009-4-RR

    Affinity and Kinetic Characterization of 83009-4-RR

    Biolayer interferometry (BLl) kinetic assays of 83009-4-RR against Human golgin 97 were performed. The affinity constant is below 1 pM.

golgin 97 Recombinant antibody

golgin 97 Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA
Cat No. 83009-4-RR

产品说明书

CloneNo. 230523A5

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, FC (Intra), ELISA

别名

GOLGA1, Golgin-97, Golgin subfamily A member 1, 230523A5

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, A549 cells, LNCaP cells, MCF-7 cells, SMMC-7721 cells
Positive IHC detected inhuman ovary cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHepG2 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:20000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83009-4-RR targets golgin 97 in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, FC (Intra), ELISA Application Description
经测试反应性 human
免疫原 golgin 97 fusion protein Ag3335 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 golgi autoantigen, golgin subfamily a, 1
别名 GOLGA1, Golgin-97, Golgin subfamily A member 1, 230523A5
计算分子量 767 aa, 88 kDa
观测分子量 97 kDa
GenBank蛋白编号BC032853
基因名称 golgin 97
Gene ID (NCBI) 2800
RRIDAB_3670753
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDQ92805
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Golgin subfamily A member 1 is a protein that in humans is encoded by the GOLGA1 gene. GOLGA1 has been shown to interact with ARL1. GOLGA1 is associated with Sjogren's syndrome. IF result shows strong Gogli staining.

实验方案

Product Specific Protocols
WB protocol for golgin 97 antibody 83009-4-RRDownload protocol
IHC protocol for golgin 97 antibody 83009-4-RRDownload protocol
IF protocol for golgin 97 antibody 83009-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols