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验证数据展示

  • WB analysis using 84818-5-RR

    WB analysis using 84818-5-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84818-5-RR (GOLIM4 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IF Staining of U2OS using 84818-5-RR

    IF Staining of U2OS using 84818-5-RR

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using GOLIM4 antibody (84818-5-RR, Clone: 242323F4 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • FC experiment of U-2 OS using 84818-5-RR

    FC experiment of U-2 OS using 84818-5-RR

    1x10^6 U2OS cells were intracellularly stained with 0.25 ug GOLIM4 Recombinant antibody (84818-5-RR, Clone:242323F4) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • FC experiment of HeLa using 84818-5-RR

    FC experiment of HeLa using 84818-5-RR

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug GOLIM4 Recombinant antibody (84818-5-RR, Clone:242323F4) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 84818-5-RR

    Affinity and Kinetic Characterization of 84818-5-RR

    Biolayer interferometry (BLl) kinetic assays of 84818-5-RR against Human GOLIM4 were performed. The affinity constant is 0.926 nM.

GOLIM4 Recombinant antibody

GOLIM4 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA
Cat No. 84818-5-RR

产品说明书

CloneNo. 242323F4

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IF/ICC, FC (Intra), ELISA

别名

P138, P 138, GPP130, GOLPH4, GIMPC

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, HepG2 cells, HEK-293 cells, MCF-7 cells, HT-1080 cells, L02 cells, C2C12 cells
Positive IF/ICC detected inU2OS cells
Positive FC (Intra) detected inU-2 OS, HeLa cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
Immunofluorescence (IF)/ICCIF/ICC : 1:250-1:1000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84818-5-RR targets GOLIM4 in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IF/ICC, FC (Intra), ELISA Application Description
经测试反应性 human, mouse
免疫原 GOLIM4 fusion protein Ag34962 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 golgi integral membrane protein 4
别名 P138, P 138, GPP130, GOLPH4, GIMPC
计算分子量82 kDa
观测分子量130-140 kDa
GenBank蛋白编号NM_014498
基因名称 GOLIM4
Gene ID (NCBI) 27333
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDO00461
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Golgi integral membrane protein 4 (GOLIM4) is a 130-kDa membrane protein that is integrated into the cis/medial Golgi apparatus, which is a kind II Golgi protein. GOLIM4 is a Golgi protein that circulates between Golgi apparatus and endosomes, and its shuttle is pH sensitive(PMID:30068697).

实验方案

Product Specific Protocols
WB protocol for GOLIM4 antibody 84818-5-RRDownload protocol
IF protocol for GOLIM4 antibody 84818-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols