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GOLPH3 Monoclonal antibody

GOLPH3 Monoclonal Antibody for WB, IF/ICC, IHC, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, Mouse, Rat and More (1)

Applications

WB, IF/ICC, IHC, ELISA and More (1)

Conjugate

Unconjugated

CloneNo.

3H2A5

Cat No : 67777-1-Ig

Print datasheet

Synonyms

Coat protein GPP34, FLJ90675, Golgi phosphoprotein 3, GOLPH3, GOPP1, GPP34, MIDAS

验证数据展示

  • WB analysis using 67777-1-Ig

    WB analysis using 67777-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67777-1-Ig (GOLPH3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis of HeLa using 67777-1-Ig

    WB analysis of HeLa using 67777-1-Ig

    WB result of GOLPH3 antibody (67777-1-Ig; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-GOLPH3 transfected HeLa cells.

  • IF Staining of HeLa using 67777-1-Ig

    IF Staining of HeLa using 67777-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using GOLPH3 antibody (67777-1-Ig, Clone: 3H2A5 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).

  • IHC staining of human colon cancer using 67777-1-Ig

    IHC staining of human colon cancer using 67777-1-Ig

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67777-1-Ig (GOLPH3 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human colon cancer using 67777-1-Ig

    IHC staining of human colon cancer using 67777-1-Ig

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67777-1-Ig (GOLPH3 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inHeLa cells, HEK-293 cellls, Jurkat cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells, 4T1 cells
Positive IHC detected inhuman colon cancer tissue, human liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells
Planning an IF experiment? We recommend our CoraLite®594 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)IF : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67777-1-Ig targets GOLPH3 in WB, IF, PLA, IHC, ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Applications WB, IF/ICC, IHC, ELISA Application Description
Cited ApplicationsPLA
Tested Reactivity Human, Mouse, Rat
Cited Reactivityhuman
Immunogen GOLPH3 fusion protein Ag5443 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name golgi phosphoprotein 3 (coat-protein)
Synonyms Coat protein GPP34, FLJ90675, Golgi phosphoprotein 3, GOLPH3, GOPP1, GPP34, MIDAS
Calculated Molecular Weight 298 aa, 34 kDa
Observed Molecular Weight33 kDa
GenBank Accession NumberBC033725
Gene Symbol GOLPH3
Gene ID (NCBI) 64083
RRIDAB_2918542
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9H4A6
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

GOLPH3 (also called GPP34, GMx33, MIDAS, or yeast Vps74p) is a 34-kDa Golgi-associated protein conserved from yeast to human. GOLPH3 binds to PtdIns(4)P-rich trans-Golgi membranes and MYO18A conveying a tensile force required for efficient tubule and vesicle formation (PMID: 19837035). GOLPH3 has been recently demonstrated as a novel oncoprotein amplified in various types of human malignancies, including melanoma, breast, non-small cell lung cancer, gliomas and connective tissue tumors (PMID:19553991; 23006319; 21499727; 22745132). Enhanced activation of mTOR signaling represents a molecular basis for the oncogenic activity of GOLPH3 (PMID: 19553991).

实验方案

Product Specific Protocols
WB protocol for GOLPH3 antibody 67777-1-IgDownload protocol
IHC protocol for GOLPH3 antibody 67777-1-IgDownload protocol
IF protocol for GOLPH3 antibody 67777-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanPLA

Cell Death Dis

GOLPH3/CKAP4 promotes metastasis and tumorigenicity by enhancing the secretion of exosomal WNT3A in non-small-cell lung cancer

Authors - Jun-Wei Song
View Article