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  • Featured Product
  • KD/KO Validated

GOLPH3 Monoclonal antibody, PBS Only

GOLPH3 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

3H2A5

Cat no : 67777-1-PBS

Print datasheet

Synonyms

MIDAS, GPP34, GOPP1, GOLPH 3, Golgi phosphoprotein 3

验证数据展示

  • WB analysis using 67777-1-Ig (same clone as 67777-1-PBS)

    WB analysis using 67777-1-Ig (same clone as 67777-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67777-1-Ig (GOLPH3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67777-1-PBS in a different storage buffer formulation.

  • WB analysis of HeLa using 67777-1-Ig (same clone as 67777-1-PBS)

    WB analysis of HeLa using 67777-1-Ig (same clone as 67777-1-PBS)

    WB result of GOLPH3 antibody (67777-1-Ig; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-GOLPH3 transfected HeLa cells. This data was developed using the same antibody clone with 67777-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 67777-1-Ig (same clone as 67777-1-PBS)

    IF Staining of HeLa using 67777-1-Ig (same clone as 67777-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using GOLPH3 antibody (67777-1-Ig, Clone: 3H2A5 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 67777-1-PBS in a different storage buffer formulation.

  • IHC staining of human colon cancer using 67777-1-Ig (same clone as 67777-1-PBS)

    IHC staining of human colon cancer using 67777-1-Ig (same clone as 67777-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67777-1-Ig (GOLPH3 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67777-1-PBS in a different storage buffer formulation.

  • IHC staining of human colon cancer using 67777-1-Ig (same clone as 67777-1-PBS)

    IHC staining of human colon cancer using 67777-1-Ig (same clone as 67777-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67777-1-Ig (GOLPH3 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67777-1-PBS in a different storage buffer formulation.

产品信息

67777-1-PBS targets GOLPH3 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen GOLPH3 fusion protein Ag5443 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name golgi phosphoprotein 3 (coat-protein)
Synonyms MIDAS, GPP34, GOPP1, GOLPH 3, Golgi phosphoprotein 3
Calculated Molecular Weight 298 aa, 34 kDa
Observed Molecular Weight34 kDa
GenBank Accession NumberBC033725
Gene Symbol GOLPH3
Gene ID (NCBI) 64083
RRIDAB_2918542
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9H4A6
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

GOLPH3 (also called GPP34, GMx33, MIDAS, or yeast Vps74p) is a 34-kDa Golgi-associated protein conserved from yeast to human. GOLPH3 binds to PtdIns(4)P-rich trans-Golgi membranes and MYO18A conveying a tensile force required for efficient tubule and vesicle formation (PMID: 19837035). GOLPH3 has been recently demonstrated as a novel oncoprotein amplified in various types of human malignancies, including melanoma, breast, non-small cell lung cancer, gliomas and connective tissue tumors (PMID:19553991; 23006319; 21499727; 22745132). Enhanced activation of mTOR signaling represents a molecular basis for the oncogenic activity of GOLPH3 (PMID: 19553991).