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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated GOLPH3 Polyclonal antibody

GOLPH3 Polyclonal Antibody for IF/ICC

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-19112

Print datasheet

Synonyms

Coat protein GPP34, FLJ90675, Golgi phosphoprotein 3, GOLPH3, GOPP1, GPP34, MIDAS

验证数据展示

  • IF Staining of HepG2 using CL488-19112

    IF Staining of HepG2 using CL488-19112

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CoraLite® Plus 488 GOLPH3 antibody (CL488-19112) at dilution of 1:200.

经过测试的应用

Positive IF/ICC detected inHepG2 cells
For other applications, we recommend the unconjugated version of this antibody, 19112-1-AP

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-19112 targets GOLPH3 in IF/ICC applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF/ICC Application Description
Tested Reactivity human, mouse, rat
Immunogen GOLPH3 fusion protein Ag5443 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name golgi phosphoprotein 3 (coat-protein)
Synonyms Coat protein GPP34, FLJ90675, Golgi phosphoprotein 3, GOLPH3, GOPP1, GPP34, MIDAS
Calculated Molecular Weight 298 aa, 34 kDa
Observed Molecular Weight 34 kDa
GenBank Accession NumberBC033725
Gene Symbol GOLPH3
Gene ID (NCBI) 64083
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9H4A6
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

GOLPH3 (also called GPP34, GMx33, MIDAS, or yeast Vps74p) is a 34-kDa Golgi-associated protein conserved from yeast to human. GOLPH3 binds to PtdIns(4)P-rich trans-Golgi membranes and MYO18A conveying a tensile force required for efficient tubule and vesicle formation (PMID: 19837035). GOLPH3 has been recently demonstrated as a novel oncoprotein amplified in various types of human malignancies, including melanoma, breast, non-small cell lung cancer, gliomas and connective tissue tumors (PMID:19553991; 23006319; 21499727; 22745132). Enhanced activation of mTOR signalling represents a molecular basis for the oncogenic activity of GOLPH3 (PMID: 19553991).

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 GOLPH3 antibody CL488-19112Download protocol
Standard Protocols
Click here to view our Standard Protocols