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GOT1 Monoclonal antibody, PBS Only

GOT1 Monoclonal Antibody for WB, IF, FC, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, chicken

Applications

WB, IF, FC, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

5A12E10

Cat No : 60317-1-PBS

Print datasheet

Synonyms

CAT, GIG18, GOT1, Transaminase A

验证数据展示

  • WB analysis using 60317-1-Ig (same clone as 60317-1-PBS)

    WB analysis using 60317-1-Ig (same clone as 60317-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 60317-1-Ig (GOT1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60317-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver cancer using 60317-1-Ig (same clone as 60317-1-PBS)

    IHC staining of human liver cancer using 60317-1-Ig (same clone as 60317-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 60317-1-Ig (GOT1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60317-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver cancer using 60317-1-Ig (same clone as 60317-1-PBS)

    IHC staining of human liver cancer using 60317-1-Ig (same clone as 60317-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 60317-1-Ig (GOT1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60317-1-PBS in a different storage buffer formulation.

  • FC experiment of HepG2 using 60317-1-Ig (same clone as 60317-1-PBS)

    FC experiment of HepG2 using 60317-1-Ig (same clone as 60317-1-PBS)

    1X10^6 HepG2 cells were stained with 0.20ug GOT1 antibody (60317-1-Ig, red) and control antibody (blue). Fixed with 90% MeOH. This data was developed using the same antibody clone with 60317-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 60317-1-Ig (same clone as 60317-1-PBS)

    IF Staining of HepG2 using 60317-1-Ig (same clone as 60317-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using GOT1 antibody (60317-1-Ig, Clone: 5A12E10 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60317-1-PBS in a different storage buffer formulation.

产品信息

60317-1-PBS targets GOT1 in WB, IF, FC, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat, chicken samples.

Tested Applications WB, IF, FC, IHC, Indirect ELISA Application Description
Tested Reactivity human, mouse, rat, chicken
Immunogen GOT1 fusion protein Ag7012 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name glutamic-oxaloacetic transaminase 1, soluble (aspartate aminotransferase 1)
Synonyms CAT, GIG18, GOT1, Transaminase A
Calculated Molecular Weight 46 kDa
Observed Molecular Weight 40-46 kDa
GenBank Accession NumberBC000498
Gene Symbol GOT1
Gene ID (NCBI) 2805
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP17174
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

背景介绍

Glutamate oxaloacetate transaminase 1 (GOT1) catalyzes the reversible reaction of L-aspartate and alpha-ketoglutarate into oxaloacetate and L-glutamate and plays a key role in carbon and nitrogen metabolism. GOT1 can potentially control the intracellular levels of reactive oxygen species (ROS) through NADPH synthesis and enhances tumor growth. GOT1 expression correlates with the growth of several tumors.