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验证数据展示

  • WB analysis using 60317-1-Ig (same clone as 60317-1-PBS)

    WB analysis using 60317-1-Ig (same clone as 60317-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 60317-1-Ig (GOT1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60317-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver cancer using 60317-1-Ig (same clone as 60317-1-PBS)

    IHC staining of human liver cancer using 60317-1-Ig (same clone as 60317-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 60317-1-Ig (GOT1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60317-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver cancer using 60317-1-Ig (same clone as 60317-1-PBS)

    IHC staining of human liver cancer using 60317-1-Ig (same clone as 60317-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 60317-1-Ig (GOT1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60317-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 60317-1-Ig (same clone as 60317-1-PBS)

    IF Staining of HepG2 using 60317-1-Ig (same clone as 60317-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using GOT1 antibody (60317-1-Ig, Clone: 5A12E10 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60317-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

GOT1 Monoclonal antibody, PBS Only

GOT1 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 60317-1-PBS

产品说明书

CloneNo. 5A12E10

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat, chicken

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

GIG18, EC:2.6.1.1, cCAT, CAT, cAspAT

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

60317-1-PBS targets GOT1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat, chicken samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat, chicken
免疫原 GOT1 fusion protein Ag7012 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 glutamic-oxaloacetic transaminase 1, soluble (aspartate aminotransferase 1)
别名 GIG18, EC:2.6.1.1, cCAT, CAT, cAspAT
计算分子量 46 kDa
观测分子量 40-46 kDa
GenBank蛋白编号BC000498
基因名称 GOT1
Gene ID (NCBI) 2805
RRIDAB_2881428
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDP17174
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Glutamate oxaloacetate transaminase 1 (GOT1) catalyzes the reversible reaction of L-aspartate and alpha-ketoglutarate into oxaloacetate and L-glutamate and plays a key role in carbon and nitrogen metabolism. GOT1 can potentially control the intracellular levels of reactive oxygen species (ROS) through NADPH synthesis and enhances tumor growth. GOT1 expression correlates with the growth of several tumors.