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验证数据展示

  • WB analysis using 60317-1-Ig

    WB analysis using 60317-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 60317-1-Ig (GOT1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver cancer using 60317-1-Ig

    IHC staining of human liver cancer using 60317-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 60317-1-Ig (GOT1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human liver cancer using 60317-1-Ig

    IHC staining of human liver cancer using 60317-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 60317-1-Ig (GOT1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HepG2 using 60317-1-Ig

    IF Staining of HepG2 using 60317-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using GOT1 antibody (60317-1-Ig, Clone: 5A12E10 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

  • Featured Product
  • KD/KO验证

GOT1 Monoclonal antibody

GOT1 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 60317-1-Ig

产品说明书

CloneNo. 5A12E10
引用文献(2)

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat, chicken

应用

WB, IHC, IF/ICC, ELISA and More (2)

别名

CAT, GIG18, GOT1, Transaminase A

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inLNCaP cells, rat brain tissue, rat heart tissue, mouse brain tissue, K-562 cells, chicken brain, chicken heart
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

60317-1-Ig targets GOT1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat, chicken samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
文献引用应用WB, IF, IP
经测试反应性 human, mouse, rat, chicken
文献引用反应性human
免疫原 GOT1 fusion protein Ag7012 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 glutamic-oxaloacetic transaminase 1, soluble (aspartate aminotransferase 1)
别名 CAT, GIG18, GOT1, Transaminase A
计算分子量 46 kDa
观测分子量 40-46 kDa
GenBank蛋白编号BC000498
基因名称 GOT1
Gene ID (NCBI) 2805
RRIDAB_2881428
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDP17174
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Glutamate oxaloacetate transaminase 1 (GOT1) catalyzes the reversible reaction of L-aspartate and alpha-ketoglutarate into oxaloacetate and L-glutamate and plays a key role in carbon and nitrogen metabolism. GOT1 can potentially control the intracellular levels of reactive oxygen species (ROS) through NADPH synthesis and enhances tumor growth. GOT1 expression correlates with the growth of several tumors.

实验方案

Product Specific Protocols
WB protocol for GOT1 antibody 60317-1-IgDownload protocol
IHC protocol for GOT1 antibody 60317-1-IgDownload protocol
IF protocol for GOT1 antibody 60317-1-IgDownload protocol
FC protocol for GOT1 antibody 60317-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

J Cell Mol Med

HIF-2α regulates non-canonical glutamine metabolism via activation of PI3K/mTORC2 pathway in human pancreatic ductal adenocarcinoma.

Authors - Wenzhu Li
View Article
humanWB,IF,IP

Nat Cell Biol

De novo pyrimidine biosynthetic complexes support cancer cell proliferation and ferroptosis defence

Authors - Chuanzhen Yang
  • KD Validated
View Article