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  • Featured Product
  • KD/KO Validated

CoraLite®594-conjugated GOT1 Monoclonal antibody

GOT1 Monoclonal Antibody for IF/ICC

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

IF/ICC

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

5A12E10

Cat no : CL594-60317

Print datasheet

Synonyms

CAT, GIG18, GOT1, Transaminase A

验证数据展示

  • IF Staining of HepG2 using CL594-60317

    IF Staining of HepG2 using CL594-60317

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CoraLite®594 GOT1 antibody (CL594-60317, Clone: 5A12E10 ) at dilution of 1:200.

  • IF Staining of HepG2 using CL594-60317

    IF Staining of HepG2 using CL594-60317

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CoraLite®594 GOT1 antibody (CL594-60317, Clone: 5A12E10 ) at dilution of 1:200.

经过测试的应用

Positive IF/ICC detected inHepG2 cells
For other applications, we recommend the unconjugated version of this antibody, 60317-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-60317 targets GOT1 in IF/ICC applications and shows reactivity with human samples.

Tested Applications IF/ICC Application Description
Tested Reactivity human
Immunogen GOT1 fusion protein Ag7012 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name glutamic-oxaloacetic transaminase 1, soluble (aspartate aminotransferase 1)
Synonyms CAT, GIG18, GOT1, Transaminase A
Calculated Molecular Weight 46 kDa
Observed Molecular Weight 40-46 kDa
GenBank Accession NumberBC000498
Gene Symbol GOT1
Gene ID (NCBI) 2805
RRIDAB_2883463
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission Maxima Wavelengths588 nm / 604 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP17174
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Glutamate oxaloacetate transaminase 1 (GOT1) catalyzes the reversible reaction of L-aspartate and alpha-ketoglutarate into oxaloacetate and L-glutamate and plays a key role in carbon and nitrogen metabolism. GOT1 can potentially control the intracellular levels of reactive oxygen species (ROS) through NADPH synthesis and enhances tumor growth. GOT1 expression correlates with the growth of several tumors.

实验方案

Product Specific Protocols
IF protocol for CL594 GOT1 antibody CL594-60317Download protocol
Standard Protocols
Click here to view our Standard Protocols