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CD42b Monoclonal antibody

CD42b Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

3G1F5

Cat no : 67857-1-Ig

Print datasheet

Synonyms

GP1BA, GP1B, CD42b alpha, BSS, Antigen CD42b-alpha

验证数据展示

  • WB analysis of human peripheral blood platelets using 67857-1-Ig

    WB analysis of human peripheral blood platelets using 67857-1-Ig

    human peripheral blood platelets were subjected to SDS PAGE followed by western blot with 67857-1-Ig (CD42b antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse spleen using 67857-1-Ig

    IHC staining of mouse spleen using 67857-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 67857-1-Ig (CD42b antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse spleen using 67857-1-Ig

    IHC staining of mouse spleen using 67857-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 67857-1-Ig (CD42b antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human placenta using 67857-1-Ig

    IHC staining of human placenta using 67857-1-Ig

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 67857-1-Ig (CD42b antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inhuman peripheral blood platelets, human blood
Positive IHC detected inmouse spleen tissue, human placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:1000-1:10000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67857-1-Ig targets CD42b in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human, mouse
Immunogen CD42b fusion protein Ag31181 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name glycoprotein Ib (platelet), alpha polypeptide
Synonyms GP1BA, GP1B, CD42b alpha, BSS, Antigen CD42b-alpha
Calculated Molecular Weight 626 aa, 69 kDa
Observed Molecular Weight145 kDa
GenBank Accession NumberBC027955
Gene Symbol CD42b
Gene ID (NCBI) 2811
ENSEMBL Gene IDENSG00000185245
RRIDAB_2918615
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP07359
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CD42b, also named as GP1BA , is a 145-kDa surface membrane protein of platelets, participates in the formation of platelet plugs by binding to the A1 domain of vWF, which is already bound to the subendothelium. It has a heterodimer form which composed of GP1BA and GP1BB.

实验方案

Product Specific Protocols
WB protocol for CD42b antibody 67857-1-IgDownload protocol
IHC protocol for CD42b antibody 67857-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols