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CoraLite® Plus 488-conjugated CD42b Monoclonal antibody

CD42b Monoclonal Antibody for WB

Host / Isotype

Mouse / IgG2b

Reactivity

Human

Applications

WB

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

3G1F5

Cat no : CL488-67857

Print datasheet

Synonyms

BSS, CD42B, CD42b alpha, GP1B, GP1BA

验证数据展示

  • WB analysis using CL488-67857

    WB analysis using CL488-67857

    Human peripheral blood platelets were subjected to SDS PAGE followed by western blot with CL488-67857 (CD42b antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inhuman peripheral blood platelets
For other applications, we recommend the unconjugated version of this antibody, 67857-1-Ig

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-67857 targets CD42b in WB applications and shows reactivity with Human samples.

Tested Applications WB Application Description
Tested Reactivity Human
Immunogen CD42b fusion protein Ag31181 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name glycoprotein Ib (platelet), alpha polypeptide
Synonyms BSS, CD42B, CD42b alpha, GP1B, GP1BA
Calculated Molecular Weight 626 aa, 69 kDa
Observed Molecular Weight145 kDa
GenBank Accession NumberBC027955
Gene Symbol CD42b
Gene ID (NCBI) 2811
ENSEMBL Gene IDENSG00000185245
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP07359
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CD42b, also named as GP1BA , is a 145-kDa surface membrane protein of platelets, participates in the formation of platelet plugs by binding to the A1 domain of vWF, which is already bound to the subendothelium. It has a heterodimer form which composed of GP1BA and GP1BB.

实验方案

Product Specific Protocols
WB protocol for CL Plus 488 CD42b antibody CL488-67857Download protocol
Standard Protocols
Click here to view our Standard Protocols