验证数据展示
经过测试的应用
| Positive WB detected in | human peripheral blood platelets |
For other applications, we recommend the unconjugated version of this antibody, 67857-1-Ig
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL488-67857 targets CD42b in WB applications and shows reactivity with Human samples.
| 经测试应用 | WB Application Description |
| 经测试反应性 | Human |
| 免疫原 | CD42b fusion protein Ag31181 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG2b |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | glycoprotein Ib (platelet), alpha polypeptide |
| 别名 | BSS, CD42B, CD42b alpha, GP1B, GP1BA |
| 计算分子量 | 626 aa, 69 kDa |
| 观测分子量 | 145 kDa |
| GenBank蛋白编号 | BC027955 |
| 基因名称 | CD42b |
| Gene ID (NCBI) | 2811 |
| ENSEMBL Gene ID | ENSG00000185245 |
| RRID | AB_2923806 |
| 偶联类型 | CoraLite® Plus 488 Fluorescent Dye |
| 最大激发/发射波长 | 493 nm / 522 nm |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | P07359 |
| 储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3 |
| 储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
CD42b, also named as GP1BA , is a 145-kDa surface membrane protein of platelets, participates in the formation of platelet plugs by binding to the A1 domain of vWF, which is already bound to the subendothelium. It has a heterodimer form which composed of GP1BA and GP1BB.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for CL Plus 488 CD42b antibody CL488-67857 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |