验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, mouse skeletal muscle tissue, mouse liver tissue |
| Positive IP detected in | mouse skeletal muscle tissue |
| Positive IHC detected in | mouse testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Planning an IHC experiment? We recommend our IHCeasy GPD2 Ready-To-Use IHC Kit. GPD2 primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
17219-1-AP targets GPD2 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IP, IHC, ELISA Application Description |
| 文献引用应用 | WB, IP |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, rat, rabbit |
| 免疫原 | GPD2 fusion protein Ag11036 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | glycerol-3-phosphate dehydrogenase 2 (mitochondrial) |
| 别名 | GDH2, GPD M, GPD2, GPDH M, GPDM, mGPDH, mtGPD |
| 计算分子量 | 41 kDa, 81 kDa |
| 观测分子量 | 68 kDa |
| GenBank蛋白编号 | BC019874 |
| 基因名称 | GPD2 |
| Gene ID (NCBI) | 2820 |
| RRID | AB_2112476 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P43304 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
GPD2(Glycerol-3-phosphate dehydrogenase, mitochondrial) belongs to the FAD-dependent glycerol-3-phosphate dehydrogenase family.It is phosphorylated only in capacitated hamster spermatozoa and is noncanonically localized in the acrosome and principal piece in human, mouse, rat, and hamster spermatozoa, though in somatic cells it is localized in the mitochondria. This noncanonical localization may imply a role of GPD2 in acrosome reaction and hyperactivation(PMID:19333995 ).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for GPD2 antibody 17219-1-AP | Download protocol |
| IHC protocol for GPD2 antibody 17219-1-AP | Download protocol |
| IP protocol for GPD2 antibody 17219-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nat Immunol Glycerol phosphate shuttle enzyme GPD2 regulates macrophage inflammatory responses.
| ||
Cell Metab Glycerol-3-phosphate biosynthesis regenerates cytosolic NAD+ to alleviate mitochondrial disease. | ||
Proc Natl Acad Sci U S A A ferroptosis defense mechanism mediated by glycerol-3-phosphate dehydrogenase 2 in mitochondria. | ||
Elife Genome-wide CRISPRi screening identifies OCIAD1 as a prohibitin client and regulatory determinant of mitochondrial Complex III assembly in human cells. | ||
Free Radic Biol Med Mitochondrial remodeling in the liver following chronic alcohol feeding to rats. | ||
J Biol Chem A refined analysis of superoxide production by mitochondrial sn-glycerol 3-phosphate dehydrogenase. |