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验证数据展示

  • WB analysis using 68174-1-Ig

    WB analysis using 68174-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68174-1-Ig (GPD2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis of HSC-T6 using 68174-1-Ig

    WB analysis of HSC-T6 using 68174-1-Ig

    HSC-T6 cells were subjected to SDS PAGE followed by western blot with 68174-1-Ig (GPD2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis using 68174-1-Ig

    WB analysis using 68174-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68174-1-Ig (GPD2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

GPD2 Monoclonal antibody

GPD2 Monoclonal Antibody for WB, ELISA
Cat No. 68174-1-Ig
CloneNo. 1C12E2

Host / Isotype

Mouse / IgG1

Reactivity

Human, Rat, Mouse

Applications

WB, ELISA

Synonyms

GDH2, GPD M, GPD2, GPDH M, GPDM, mGPDH, mtGPD

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive WB detected inLNCaP cells, HSC-T6 cells, NIH/3T3 cells, HeLa cells, 4T1 cells
Planning an IHC experiment? We recommend our IHCeasy GPD2 Ready-To-Use IHC Kit. GPD2 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68174-1-Ig targets GPD2 in WB, ELISA applications and shows reactivity with Human, Rat, Mouse samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity Human, Rat, Mouse
Immunogen GPD2 fusion protein Ag11212 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name glycerol-3-phosphate dehydrogenase 2 (mitochondrial)
Synonyms GDH2, GPD M, GPD2, GPDH M, GPDM, mGPDH, mtGPD
Calculated Molecular Weight 41 kDa, 81 kDa
GenBank Accession NumberBC019874
Gene Symbol GPD2
Gene ID (NCBI) 2820
RRIDAB_3085046
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP43304
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

GPD2(Glycerol-3-phosphate dehydrogenase, mitochondrial) belongs to the FAD-dependent glycerol-3-phosphate dehydrogenase family.It is phosphorylated only in capacitated hamster spermatozoa and is noncanonically localized in the acrosome and principal piece in human, mouse, rat, and hamster spermatozoa, though in somatic cells it is localized in the mitochondria. This noncanonical localization may imply a role of GPD2 in acrosome reaction and hyperactivation(PMID:19333995 ).

实验方案

Product Specific Protocols
WB protocol for GPD2 antibody 68174-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols