验证数据展示
经过测试的应用
Positive WB detected in | LNCaP cells, HSC-T6 cells, NIH/3T3 cells, HeLa cells, 4T1 cells |
推荐稀释比
Application | Dilution |
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Western Blot (WB) | WB : 1:5000-1:50000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
68174-1-Ig targets GPD2 in WB, ELISA applications and shows reactivity with Human, Rat, Mouse samples.
Tested Applications | WB, ELISA Application Description |
Tested Reactivity | Human, Rat, Mouse |
Immunogen | GPD2 fusion protein Ag11212 种属同源性预测 |
Host / Isotype | Mouse / IgG1 |
Class | Monoclonal |
Type | Antibody |
Full Name | glycerol-3-phosphate dehydrogenase 2 (mitochondrial) |
Synonyms | GDH2, GPD M, GPD2, GPDH M, GPDM, mGPDH, mtGPD |
Calculated Molecular Weight | 41 kDa, 81 kDa |
GenBank Accession Number | BC019874 |
Gene Symbol | GPD2 |
Gene ID (NCBI) | 2820 |
RRID | AB_3085046 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein G purification |
UNIPROT ID | P43304 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
GPD2(Glycerol-3-phosphate dehydrogenase, mitochondrial) belongs to the FAD-dependent glycerol-3-phosphate dehydrogenase family.It is phosphorylated only in capacitated hamster spermatozoa and is noncanonically localized in the acrosome and principal piece in human, mouse, rat, and hamster spermatozoa, though in somatic cells it is localized in the mitochondria. This noncanonical localization may imply a role of GPD2 in acrosome reaction and hyperactivation(PMID:19333995 ).
实验方案
Product Specific Protocols | |
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WB protocol for GPD2 antibody 68174-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |