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验证数据展示

  • WB analysis using 68174-1-Ig (same clone as 68174-1-PBS)

    WB analysis using 68174-1-Ig (same clone as 68174-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68174-1-Ig (GPD2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68174-1-PBS in a different storage buffer formulation.

  • WB analysis of HSC-T6 using 68174-1-Ig (same clone as 68174-1-PBS)

    WB analysis of HSC-T6 using 68174-1-Ig (same clone as 68174-1-PBS)

    HSC-T6 cells were subjected to SDS PAGE followed by western blot with 68174-1-Ig (GPD2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68174-1-PBS in a different storage buffer formulation.

  • WB analysis using 68174-1-Ig (same clone as 68174-1-PBS)

    WB analysis using 68174-1-Ig (same clone as 68174-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68174-1-Ig (GPD2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68174-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP50432-1

    Cytometric bead array standard curve of MP50432-1

    Cytometric bead array standard curve of MP50432-1, GPD2 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 68174-2-PBS. Detection antibody: 68174-1-PBS. Standard:Ag11212. Range: 0.391-200 ng/mL.

GPD2 Monoclonal antibody, PBS Only (Detector)

GPD2 Monoclonal Antibody for WB, Cytometric bead array, Indirect ELISA
Cat No. 68174-1-PBS

产品说明书

CloneNo. 1C12E2

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat

应用

WB, Cytometric bead array, Indirect ELISA

别名

GPD M, GPD 2, Glycerol-3-phosphate dehydrogenase, mitochondrial, GDH2, EC:1.1.5.3

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy GPD2 Ready-To-Use IHC Kit. GPD2 primary antibody included.

产品信息

68174-1-PBS targets GPD2 as part of a matched antibody pair:

MP50432-1: 68174-2-PBS capture and 68174-1-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, Cytometric bead array, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 GPD2 fusion protein Ag11212 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 glycerol-3-phosphate dehydrogenase 2 (mitochondrial)
别名 GPD M, GPD 2, Glycerol-3-phosphate dehydrogenase, mitochondrial, GDH2, EC:1.1.5.3
计算分子量 41 kDa, 81 kDa
GenBank蛋白编号BC019874
基因名称 GPD2
Gene ID (NCBI) 2820
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDP43304
储存缓冲液 PBS Only
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

GPD2(Glycerol-3-phosphate dehydrogenase, mitochondrial) belongs to the FAD-dependent glycerol-3-phosphate dehydrogenase family.It is phosphorylated only in capacitated hamster spermatozoa and is noncanonically localized in the acrosome and principal piece in human, mouse, rat, and hamster spermatozoa, though in somatic cells it is localized in the mitochondria. This noncanonical localization may imply a role of GPD2 in acrosome reaction and hyperactivation(PMID:19333995 ).