验证数据展示
经过测试的应用
| Positive IF/ICC detected in | PC-3 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL594-67178 targets GPI in IF/ICC applications and shows reactivity with Human samples.
| 经测试应用 | IF/ICC Application Description |
| 经测试反应性 | Human |
| 免疫原 | GPI fusion protein Ag7419 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | glucose phosphate isomerase |
| 别名 | AMF, Autocrine motility factor, Glucose 6 phosphate isomerase, glucose phosphate isomerase, GNPI, GPI, Neuroleukin, NLK, PGI, PHI, Phosphoglucose isomerase, Phosphohexose isomerase, SA 36, Sperm antigen 36 |
| 计算分子量 | 63 kDa |
| 观测分子量 | 55-64 kDa |
| GenBank蛋白编号 | BC004982 |
| 基因名称 | GPI |
| Gene ID (NCBI) | 2821 |
| RRID | AB_3084756 |
| 偶联类型 | CoraLite® Plus 594 Fluorescent Dye |
| 最大激发/发射波长 | 594 nm / 615 nm |
| 形式 | Liquid |
| 纯化方式 | Protein G purification |
| UNIPROT ID | P06744 |
| 储存缓冲液 | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| 储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
GPI(Glucose-6-phosphate isomerase), which is also named as autocrine motility factor (AMF), phosphoglucose isomerase(PGI), Neuroleukinis(NLK), phosphohexose isomerase(PHI) or sperm antigen 36(SA-36), is a housekeeping cytosolic enzyme that plays a key role in both glycolysis and gluconeogenesis pathways. It is also a multifunctional protein that displays cytokine properties, eliciting mitogenic, motogenic, and differentiation activities, and has been implicated in tumor progression and metastasis (PMID:12783864, 19603112). This protein can exsit as a homodimer in the catalytically active form and a monomer in the secreted form (PMID:11371164). It has 2 isoforms produced by alternative splicing with the calculated molecular mass of 63-64kDa, and an apparent molecular mass of 55 and 64 kDa under non-reducing and reducing conditions, respectively(PMID: 19603112, 11004567).
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 594 GPI antibody CL594-67178 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |